Biosensor Response Electrode Electric Field High Salt
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Solution Overview
Problem
Existing biosensors face challenges in accurately measuring DNA or RNA concentrations in test solutions with high salt concentrations due to screening effects, requiring dilution which reduces detection limit and sensitivity.
Innovation Solution
A sensing device comprising a transistor with a response electrode and a receptor, where the response electrode is positioned opposite to the transistor's gate end and spaced apart, generating an electric field of at least 1 millivolt/centimeter, allowing for low detection limits and high sensitivity by enabling specific binding of the receptor with the target substance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the test solution is diluted to reduce screening effect, then measurement accuracy is improved, but detection limit and sensitivity deteriorate
Solution Approach 1:
The patent applies parameter changes by introducing an electric field (F≥1 mV/cm) between the response electrode and gate end to counteract the screening effect of high salt concentrations. This allows measurements to be performed on undiluted or minimally diluted samples, maintaining both measurement accuracy and detection limit without requiring excessive dilution.
2Quantity of substance
If high salt concentration test solution is used directly, then detection sensitivity is maintained, but screening effect interferes with measurement
Solution Approach 1:
The patent converts the harmful screening effect into a controllable parameter by applying an external electric field that compensates for the salt concentration effects. This allows the biosensor to operate directly in high salt concentration environments (such as blood) without dilution, maintaining detection sensitivity while eliminating the interference of the screening effect through the applied field.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device achieves low detection limits and high sensitivity, enabling accurate measurement of DNA or RNA concentrations even in high salt concentrations, with the receptor's specific binding providing high selectivity for the target substance.
Implementation Method 1
When a voltage is applied to the at least one response electrode, an electric field between the at least one response electrode and the gate end of the transistor is F, and F≥1 millivolt/centimeter
Data Source
AI summary
A sensing device including a transistor, at least one response electrode, and a receptor is provided. The transistor includes a gate end, a source end, a drain end, and a semiconductor layer. The source end and the drain end are located on the semiconductor layer, and the gate end is located between the source end and the drain end. The at least one response electrode is disposed opposite to the gate end of the transistor and spaced apart from the transistor. The receptor is bonded onto the at least one response electrode. When a voltage is applied to the at least one response electrode, an electric field between the at least one response electrode and the gate end of the transistor is F, and F≥1 mV/cm.