Electrochemical Biosensor Self-Calibration for Enzyme Variation
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Solution Overview
Problem
Existing electrochemical biosensors face variations in measured values due to enzyme activity and enzyme amount, leading to inaccurate substance concentration measurements.
Innovation Solution
A self-calibration method is implemented using a biosensor with an electrochemical measurement cell, applying first and second voltages to measure signals related to oxidoreductase activity and substance concentration, with correction coefficients calculated to accurately determine substance concentration.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If a charge transfer limiting current measurement method is used to reduce measurement time and cost, then productivity is improved, but measurement precision deteriorates due to variations in enzyme activity and enzyme amount
Solution Approach 1:
The patent applies a preliminary calibration step before actual measurement. A calibration curve is created by measuring known concentrations of the target substance, establishing the relationship between current signal and concentration for that specific sensor. This preliminary action accounts for variations in enzyme activity and amount, enabling accurate subsequent measurements without requiring longer measurement times.
2Measurement precision
If sensor calibration is performed for each production lot to improve measurement precision, then measurement accuracy is improved, but device complexity increases
Solution Approach 1:
The patent implements a self-calibration approach where each sensor performs its own calibration by measuring known standard solutions and generating its own calibration curve. The sensor system automatically determines the relationship between current and concentration without requiring external calibration equipment or complex manual procedures. This self-service mechanism maintains high measurement precision while minimizing device complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces variation in measured values across multiple sensors, enhancing measurement accuracy and allowing for individual sensor calibration, improving the precision of substance concentration analysis.
Implementation Method 1
an oxidoreductase which is capable of catalyzing an oxidation reduction reaction and contributing to an electron transfer to and from the electrode
Implementation Method 2
the concentration of a substance is measured by detecting a current resulting from the charge transfer process
Data Source
AI summary
Provided is a measuring method using a biosensor, the method including: introducing a sample containing a substance to be measured into an electrochemical measurement cell, wherein the electrochemical measurement cell comprises: an insulating base plate; and an electrode system applying a first voltage to the electrode system; applying a second voltage to the electrode system; obtaining a first signal; obtaining a second signal; and correcting the second signal by the first signal, to determine the concentration of the substance to be measured in the sample.