Biosensor Simultaneous Detection and Biological Activity Measurement
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional methods for detecting substances interacting with physiologically active substances on biosensors do not allow for the simultaneous measurement of the biological activity of these substances, limiting the analysis of the effects of interacting substances on their biological activity.
Innovation Solution
A biosensor method that immobilizes physiologically active substances on a substrate, enabling both the detection of interacting substances and the measurement of biological activity using surface plasmon resonance analysis, allowing for the analysis of the effects of test substances on the biological activity of the physiologically active substances.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional SPR measurement methods are used to detect substances interacting with physiologically active substances, then detection capability is achieved, but the ability to simultaneously measure biological activity is lost
Solution Approach 1:
The measurement chip is designed to perform multiple functions: it can detect substances interacting with physiologically active substances through SPR signal changes, and simultaneously measure the biological activity of the physiologically active substances by allowing reaction with specific reagents. This multi-functional design resolves the contradiction by enabling both detection capabilities in a single system.
2Productivity
If a single biosensor is used for both detection and biological activity measurement, then measurement efficiency is improved, but measurement complexity increases
Solution Approach 1:
The measurement chip is divided into distinct functional regions: a first region for detecting substance interaction with the physiologically active substance, and a second region for measuring biological activity through reaction with reagents. This segmentation allows simultaneous multi-parameter measurement while maintaining clear functional separation, thus improving efficiency without excessive complexity.
3Loss of information
If multiple measurement techniques are used to analyze both interaction and biological activity, then measurement comprehensiveness is improved, but operational complexity increases
Solution Approach 1:
The invention merges interaction detection and biological activity measurement into a single integrated measurement chip. The chip combines SPR-based detection of substance binding with simultaneous measurement of biological activity through on-chip reactions, eliminating the need for separate measurement systems and reducing operational complexity while maintaining comprehensive measurement capability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the determination of whether test substances bind to the active site of physiologically active substances and assesses their impact on biological activity, facilitating the screening of test substances and improving interaction measurement capabilities.
Implementation Method 1
The SPR measurement technique is a method of measuring changes in the refractive index near an organic functional film attached to the metal film of a chip by measuring a peak shift in the wavelength of reflected light
Implementation Method 2
The OCM measurement technique is a technique of detecting adsorbed or desorbed mass at the ng level, using a change in frequency of a crystal due to adsorption or desorption of a substance on gold electrodes of a quartz crystal
Data Source
AI summary
It is an object of the present invention to provide a method for simultaneously carrying out the detection or measurement of a substance interacting with a physiologically active substance immobilized on a biosensor and the analysis of the effects of the above substance on the biological activity of the physiologically active substance. The present invention provides a method using a biosensor formed by immobilizing a physiologically active substance on a substrate, wherein the detection and measurement of a substance interacting with said physiologically active substance and the measurement of the biological activity of said physiologically active substance are carried out on said single biosensor.