Biosensor Substrate Dual-Mode SPR and Spectroscopic Activity Measurement
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Solution Overview
Problem
Conventional surface plasmon resonance (SPR) measurement techniques fail to measure the biological activity of physiologically active substances immobilized on biosensor substrates, limiting the analysis of interactions with test substances as the biological activity is not maintained in these states.
Innovation Solution
A method that measures both the amount and biological activity of physiologically active substances on a single biosensor substrate using surface plasmon resonance analysis and spectroscopic measurements, allowing for the calculation of biological activity per unit quantity and optimization of immobilization methods to maintain activity during interaction detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional SPR measurement techniques are used to detect binding amount changes, then high sensitivity detection is achieved, but biological activity measurement is not possible
Solution Approach 1:
The measurement process is divided into two separate measurement steps: first measuring the binding amount of the physiologically active substance using SPR, then measuring its biological activity using spectroscopic methods. This segmentation allows each measurement to be optimized for its specific purpose while using the same biosensor substrate.
Solution Approach 2:
The patent uses spectroscopic methods as an intermediary measurement technique that can detect biological activity without interfering with the SPR-based binding amount measurement. This intermediary approach enables dual measurement capabilities on the same substrate.
2Adaptability or versatility
If physiologically active substances are immobilized on biosensor substrate, then interaction detection is enabled, but biological activity is not maintained
Solution Approach 1:
The patent performs preliminary measurements of both binding amount and biological activity before conducting interaction detection experiments. This preliminary characterization allows optimization of immobilization conditions to maintain biological activity while enabling interaction detection.
Solution Approach 2:
The measurement results of biological activity serve as feedback to evaluate and optimize the immobilization method. By monitoring biological activity levels, the immobilization conditions can be adjusted to maintain activity while preserving interaction detection capability.
3Productivity
If single biosensor substrate is used for both measurements, then measurement efficiency is improved, but measurement complexity increases
Solution Approach 1:
The patent makes the biosensor substrate multi-functional by enabling it to serve both SPR-based binding amount measurement and spectroscopic biological activity measurement. This universality eliminates the need for separate substrates and reduces overall measurement complexity despite the dual measurement capability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate measurement of biological activity and interaction with test substances, ensuring that the physiologically active substances are immobilized in an active state, thereby improving the specificity and reliability of the interaction analysis.
Implementation Method 1
The SPR measurement technique is a method of measuring changes in the refractive index near an organic functional film attached to the metal film of a chip by measuring a peak shift in the wavelength of reflected light
Implementation Method 2
The QCM measurement technique is a technique of detecting adsorbed or desorbed mass at the ng level, using a change in frequency of a crystal due to adsorption or desorption of a substance on gold electrodes of a quartz crystal
Data Source
AI summary
It is an object of the present invention to provide a method for carrying out on a same biosensor both of the measurement of the amount of a physiologically active substance immobilized on a biosensor substrate and the measurement of the biological activity of the above physiologically active substance, thereby analyzing the interaction of a substance with the above physiologically active substance that maintains its activity. The present invention provides a measurement method using a biosensor substrate, wherein both the amount of a physiologically active substance immobilized on the substrate and the biological activity of the physiologically active substance immobilized on the substrate are measured on said same biosensor substrate.