Biosensor Substrate Modification via Ethanol Treatment
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Solution Overview
Problem
The manufacturing process of conventional optical biosensors requires strong acid or alkali solutions, leading to hazardous working conditions and environmental pollution, as well as high costs due to the need for specialized equipment like oxygen plasma cleaners.
Innovation Solution
A method using an ethanol solution to create negative charges on silicon-containing substrates, eliminating the need for strong acid or alkali solutions and specialized instruments, thereby improving safety and reducing manufacturing costs by forming active polymer layers and coupling capture biomolecules without the use of these hazardous materials.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If strong acid solution or strong alkali solution is used to form negative charges on substrate surface, then negative charges are formed enabling electrostatic bonding, but working conditions become hazardous and environmental pollution occurs
Solution Approach 1:
The patent changes the chemical parameters of the treatment solution from strong acid/alkali to a milder solution (e.g., sodium hydroxide at controlled concentration or alternative treatments), maintaining the ability to form negative charges on the substrate surface while reducing the harmful effects. This parameter modification allows the process to remain effective for creating electrostatic bonding capability without the severe safety and environmental issues of conventional strong chemicals.
2Reliability
If oxygen plasma treatment is used to form negative charges on substrate surface, then negative charges are formed, but specialized instruments and high cost are required
Solution Approach 1:
The patent replaces expensive, complex oxygen plasma treatment equipment with simpler, more affordable chemical treatment methods. Instead of requiring specialized plasma generators and vacuum systems, the invention uses conventional chemical solutions that can be applied with standard laboratory equipment, dramatically reducing the manufacturing cost and device complexity while achieving the same functional outcome of forming negative charges on the substrate.
Solution Approach 2:
The patent substitutes the mechanical/physical plasma treatment system with a chemical solution-based approach. Rather than using high-energy plasma physics to modify the substrate surface, the invention employs chemical reactions in solution to achieve the same surface charge modification, replacing complex physical systems with simpler chemical processes.
3Reliability
If conventional manufacturing method with strong acid/alkali is used, then biosensor functionality is achieved, but waste disposal risks and environmental pollution increase
Solution Approach 1:
The patent modifies the chemical parameters of the manufacturing process by using milder solutions instead of strong acids/alkalis, which reduces the harmfulness of the waste generated. The altered chemical parameters enable the same functional outcome in terms of biosensor performance while producing waste that is safer to handle and dispose of, thereby reducing environmental pollution risks.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances safety, reduces waste disposal risks, and lowers production costs while maintaining effective biosensor functionality, allowing for stable and efficient biomolecule coupling and improved detection specificity and sensitivity.
Implementation Method 1
forming negative charges on at least one surface of a silicon-containing substrate by an ethanol solution
Implementation Method 2
An active polymer layer with positive charges is then electrostatically bond to the surface of the silicon-containing substrate
Implementation Method 3
an antibody with negative charges can electrostatically bond to the surface of the polymer active polymer layer
Data Source
AI summary
A method for manufacturing a glass-based biosensor is used to solve the problem of the use of a solution containing a strong acid or a strong base or of an oxygen plasma treatment. The method comprises modifying a silicon-containing substrate by an alcohol solution to form negative charges on at least one coupling surface of the silicon-containing substrate. A least one active layer of polymer having positive charges is formed on the at least one surface of the silicon-containing substrate, respectively. Each of the at least one active layer of polymer has a coupling surface and an active surface opposite to the coupling surface, and the at least one active layer of polymer couples to the silicon-containing substrate via the coupling surface. A plurality of capture biomolecules couples to the active surface.