Bioengineered Bladder Patch Using Decellularized Amnion
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Solution Overview
Problem
Current biomaterials for bladder tissue repair, such as polyglycolic acid and porcine bladder matrix, face challenges with immunogenicity and rejection, and lack success in recellularization, making them unsuitable for durable and effective bladder reconstruction, especially for conditions like vesicovaginal fistula.
Innovation Solution
A bioengineered regenerative cell-seeded multiphasic bladder patch is developed using electrochemically compacted collagen and human amniotic membrane, which provides structural integrity and biochemical cues for cell attachment and proliferation, avoiding extensive dissection and suturing, and is safe near ureteral orifices.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Strength
If conventional biomaterials (polyglycolic acid, porcine bladder matrix) are used for bladder tissue repair, then structural support is provided, but immunogenicity and rejection occur leading to poor recellularization
Solution Approach 1:
The patent extracts and removes immunogenic components from the biomaterial system by using decellularized amnion tissue, which eliminates cellular antigens that cause rejection while retaining the beneficial extracellular matrix structure for structural support and recellularization
Solution Approach 2:
The patent creates a composite tissue engineering construct combining decellularized amnion tissue with seeded epithelial cells, forming a hybrid material that provides both structural integrity and immunocompatibility while enabling functional recellularization
2Reliability
If standard surgical repair of vesicovaginal fistula is performed, then fistula closure is achieved, but extensive dissection and suturing are required increasing surgical complexity
Solution Approach 1:
The patent applies preliminary action by pre-seeding the amnion tissue scaffold with epithelial cells before implantation, so that the tissue is already populated with functional cells ready for integration, eliminating the need for extensive surgical dissection and suturing procedures
Solution Approach 2:
The patent enables self-service by designing a scaffold that spontaneously integrates and heals the fistula defect through its bioactive properties and pre-seeded cells, allowing the tissue to self-organize and close the defect without requiring complex surgical intervention
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The patch supports epithelial cell attachment, survival, and increased proliferation, offering a durable and effective solution for bladder reconstruction with reduced risk of ureteral injury and improved healing rates.
Implementation Method 1
Electrochemically compacted collagen sheets were fabricated using planar electrodes
Implementation Method 2
crosslinked using genipin (2% genipin in 90% ethanol)
Implementation Method 3
adhered to a genipin crosslinked collagen sheet/PCL using the gluing solution containing sodium hyaluronate, bovine serum albumin and dextran
Data Source
AI summary
A tissue repair patch having an outer side and an inner side is described. The tissue repair patch includes a structural component comprising collagen and/or chorion and a regenerative component comprising amniotic tissue. Methods of tissue repair using the tissue repair patch are also described.