Blind Deconvolution of Flow Cytometer Particle Emission
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current flow cytometer systems face challenges in accurately determining the emission of individual particles due to geometrical convolution of excitation and emission characteristics, leading to inaccurate and noisy results, especially when particle size and velocity vary.
Innovation Solution
A method and system for blind deconvolution of flow cytometer particle emissions using a processor to calculate total fluorescent emission by determining the extent of geometrical convolution and fitting time samples to a best-fit function, accounting for particle size and velocity, and sampling at fixed intervals without a sheath fluid.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional flow cytometry detection is used, then particle emission can be detected, but measurement precision deteriorates due to geometrical convolution and noise
Solution Approach 1:
The patent introduces a sheath fluid as an intermediary medium that surrounds the particle and provides a uniform refractive index environment. This mediator reduces optical distortion and scattering at particle boundaries, thereby improving measurement precision and reducing noise in the detected emission signal
Solution Approach 2:
The system uses a composite optical detection approach combining multiple detection angles and wavelengths. By collecting emission signals from multiple directions and spectral regions, the system creates a composite measurement that compensates for geometrical convolution effects and improves overall detection accuracy
2Device complexity
If flow cytometry is performed without sheath fluid, then device complexity is reduced, but measurement precision deteriorates due to particle velocity and size variations
Solution Approach 1:
The patent implements dynamic adjustment of detection parameters based on real-time particle characteristics. The system adapts sampling rates, detection angles, and integration times according to particle velocity and size variations, maintaining measurement precision without requiring complex sheath fluid delivery systems
Solution Approach 2:
The system changes detection parameters such as sampling frequency, integration window, and detection angle dynamically based on particle properties. This allows accurate emission quantification across varying particle sizes and velocities without adding sheath fluid complexity
3Measurement precision
If multiple detection parameters are used, then measurement precision improves, but device complexity increases
Solution Approach 1:
The patent designs a multi-functional detection apparatus where a single optical system performs multiple functions: detecting emission intensity, determining particle size, and measuring velocity. By making the detection system universal, it achieves high measurement precision without proportionally increasing device complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for precise determination of individual particle emissions, improving signal-to-background ratio and accurately quantifying particle emissions regardless of particle size and velocity, providing a more reliable analysis compared to prior art methods.
Implementation Method 1
the particle being adapted to be irradiated by the apparatus and to emit at least one fluorescent emission responsive to the laser excitation energy
Data Source
Figure 1~2
Figure 3~5
Figure 6~7A
AI summary
A method and system for determining a total particle emission of a flow cytometer particle, the system including an apparatus adapted to pass a laser excitation energy into a flow cytometer channel through which a particle passes, the particle being adapted to be irradiated by the apparatus and to emit fluorescent emission responsive to the laser excitation energy, wherein the fluorescent emission results from a geometrical convolution of a geometrical form of both the laser excitation energy and a geometric characteristic of fluorescent emission of the particle, an analog emission detector adapted to detect at least some of the fluorescent emission and to output an analog electrical emission, a digital sampler adapted to provide multiple time samples of the analog electrical emission, and a processor, adapted to process the multiple time samples and to calculate a total fluorescent emission of said particle, by determining the extent of said geometric convolution and fitting said time samples to a function of a same extent.