Blood Aβ Peptide Measurement With Basic Organic-Solvent Release
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Solution Overview
Problem
Existing methods for measuring amyloid β (Aβ) peptide in blood samples are inaccurate due to low concentrations and high carryover issues when using acidic solutions for dissociating the peptide from antibodies, leading to difficulties in quantitative analysis.
Innovation Solution
A method involving the use of a basic solution containing an organic solvent to release Aβ peptide from its antibody complex, followed by mass spectrometry for accurate measurement, which reduces carryover and maintains sensitivity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If an acidic solution is used to release Aβ peptide from antibody complex, then the peptide can be dissociated, but carryover increases and measurement accuracy deteriorates
Solution Approach 1:
The patent changes the pH parameter from acidic to basic (pH 10-14) to release the Aβ peptide from the antibody complex. This parameter change simultaneously reduces carryover and maintains measurement accuracy, resolving the technical contradiction between measurement precision and harmful factors.
Solution Approach 2:
Instead of using the conventional acidic solution to release the peptide, the patent inverts the approach by using a basic solution. This inversion eliminates the carryover problem while maintaining effective peptide dissociation, thereby improving measurement accuracy.
2Ease of operation
If blood sample is used instead of CSF, then collection burden is reduced, but Aβ peptide concentration decreases making accurate measurement difficult
Solution Approach 1:
The patent employs parameter changes including pH adjustment to 10-14 and addition of organic solvents to enhance the recovery and detection sensitivity of low-concentration Aβ peptide in blood samples, enabling accurate measurement despite the lower concentration compared to CSF.
Solution Approach 2:
The patent replaces conventional measurement approaches with mass spectrometry-based detection, which provides superior sensitivity and accuracy for measuring low-concentration Aβ peptide in blood samples, thereby maintaining measurement precision while using the easier-to-collect blood sample.
3Measurement precision
If conventional measurement methods are used for low concentration Aβ peptide in blood, then standard procedures are followed, but measurement accuracy and sensitivity are insufficient
Solution Approach 1:
The patent applies parameter changes including pH adjustment to 10-14 and organic solvent addition to optimize the release and detection of Aβ peptide, significantly enhancing both measurement accuracy and sensitivity for low-concentration samples.
Solution Approach 2:
The patent substitutes conventional measurement methods with mass spectrometry, which provides superior detection sensitivity and accuracy for low-concentration Aβ peptide, simultaneously improving both measurement precision and productivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method allows for precise and sensitive quantification of Aβ peptide in blood samples without the need for solvent exchange, overcoming the limitations of acidic solutions and enhancing measurement accuracy.
Implementation Method 1
releasing the Aβ peptide from the complex with a basic solution containing an organic solvent
Implementation Method 2
measuring the released Aβ peptide by mass spectrometry
Data Source
AI summary
Disclosed is a method for measuring an Aβ peptide, comprising: mixing a blood sample comprising an Aβ peptide and an antibody that specifically binds to the Aβ peptide to form a complex of the Aβ peptide and the antibody; releasing the AP peptide from the complex with a basic solution comprising an organic solvent; and measuring the released Aβ peptide by mass spectrometry.