Blood Analysis Kit Using Normal Component Dilution Factor
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Solution Overview
Problem
Current blood analysis methods face challenges in achieving high repeatability and reproducibility when using small blood samples, particularly with volumes of 50 μL or less, due to limitations in dilution factors and the accuracy of measurement values, leading to decreased reliability and increased measurement errors.
Innovation Solution
A blood analysis method and kit that utilize a diluent solution with a dilution factor of 14 times or more for blood plasma components, using sodium ions or chloride ions as normal components homeostatically present in blood, to accurately determine the concentration of target components in small blood samples by defining the amount of normal components derived from the diluent solution or kit members, thereby enhancing measurement accuracy and reliability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If a high dilution factor is used to analyze small blood samples, then the volume of blood required is reduced, but the measurement precision and repeatability deteriorate
Solution Approach 1:
The patent changes the parameter of dilution factor to achieve both small blood sample volume and high measurement precision. By optimizing the dilution factor within a specific range (5-20 times), the method enables accurate measurement of target components in small blood samples while maintaining repeatability and reproducibility, resolving the contradiction between reduced sample volume and maintained measurement precision
Solution Approach 2:
The patent introduces an intermediary substance (indicator substance or internal standard) to bridge the measurement between diluted blood samples and target components. This intermediary enables accurate quantification even at high dilution factors, allowing small blood volumes to be analyzed with maintained precision through the intermediary's concentration changes
2Quantity of substance
If a high dilution factor is used to analyze small blood samples, then the blood volume required is reduced, but the repeatability and reproducibility of measurement values decrease
Solution Approach 1:
The patent incorporates feedback mechanisms through the use of indicator substances or internal standards that provide reference values for measurement validation. This feedback loop allows continuous monitoring and adjustment of measurement processes, ensuring repeatability and reproducibility even when high dilution factors are applied to small blood samples
Solution Approach 2:
By optimizing the dilution factor parameter within the range of 5-20 times, the patent achieves the best balance between reduced blood volume and maintained measurement reliability. This parameter optimization ensures that both repeatability and reproducibility are preserved while enabling analysis of small blood samples
3Measurement precision
If the dilution factor is increased to analyze small blood samples, then the measurement accuracy improves, but the complexity of the analysis process increases
Solution Approach 1:
The patent uses an intermediary substance (indicator or internal standard) that simplifies the measurement process by providing a reference for calculation. This intermediary enables accurate concentration determination of target components in diluted samples through straightforward ratio calculations, reducing the complexity of high-dilution analysis while maintaining precision
Solution Approach 2:
The patent creates a simplified measurement model by using indicator substances that replicate the behavior of target components in the diluted sample. This copying approach allows accurate measurement through proportional relationships, reducing the complexity of analyzing small blood samples at high dilution factors
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves high accuracy and reliability in analyzing target components in small blood samples by precisely calculating the dilution factor, maintaining error accuracy within 5% or less, even with small blood volumes, thereby improving the repeatability and reproducibility of measurement values.
Implementation Method 1
a step of diluting a collected blood sample with a diluent solution; a step of determining a dilution factor by using a normal value of a normal component
Implementation Method 2
a separation instrument for separating and recovering blood plasma from the blood sample diluted with the diluent solution
Data Source
AI summary
An object of the present invention is to provide a blood analysis method and a blood test kit, which are for performing quantitative analysis of components by precisely obtaining a dilution factor. According to the present invention, provided is a blood analysis method including a step of diluting a collected blood sample with a diluent solution; a step of determining a dilution factor by using a normal value of a normal component which is homeostatically present in blood; and a step of analyzing a concentration of a target component in the blood sample, in which the blood analysis method uses a member selected from the group consisting of a first storing instrument for storing a diluent solution, a separation instrument for separating and recovering blood plasma from the blood sample diluted with the diluent solution, a holding instrument for holding the separation instrument, a second storing instrument for storing the recovered blood plasma, and a sealing instrument for keeping the stored blood plasma within the second storing instrument, in which the diluent solution defines an amount of the normal component which is derived from the diluent solution and/or the members and may be contained in the diluent solution, and in which a volume of the blood sample is 50 μL or less, and a dilution factor of a blood plasma component in the blood sample is 14 times or more.