Blood Analysis Method Correcting Dilution Factor for Hemolysis
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Solution Overview
Problem
Existing blood analysis methods face challenges in accurately measuring multiple target components from small blood samples, particularly when hemolysis occurs, leading to errors in concentration measurements due to differences in normal component concentrations between blood cells and plasma.
Innovation Solution
A blood analysis method that involves diluting the blood sample with a diluent solution, measuring the amount of hemolysis, and correcting the dilution factor using a normal component homeostatically present in blood, such as sodium ions, to accurately determine the concentration of target components in blood plasma.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If a small volume of blood sample is used for analysis, then the invasiveness of blood collection is reduced and patient comfort is improved, but the accuracy of concentration measurements deteriorates due to hemolysis-induced errors
Solution Approach 1:
The patent implements a feedback mechanism by measuring the concentration of a normal component (such as sodium ions) in the diluted sample, comparing it to the known pre-dilution concentration, and using this information to calculate and correct the dilution factor. This feedback loop compensates for hemolysis-induced errors and maintains measurement accuracy even when using small blood sample volumes.
Solution Approach 2:
The patent changes the parameter used for dilution factor calculation from direct volume measurement to concentration-based calculation. By measuring the concentration of a normal component after dilution and using it to derive the dilution factor, the method becomes insensitive to hemolysis and enables accurate measurements from small blood volumes.
2Ease of manufacture
If the dilution factor is calculated based on the volume of diluent solution added, then the calculation is simple, but the accuracy deteriorates when hemolysis occurs because the normal component concentration changes
Solution Approach 1:
Instead of relying on simple volume-based calculation, the patent introduces feedback by measuring the actual concentration of a normal component in the diluted sample. This measured concentration is used to calculate the true dilution factor, compensating for hemolysis effects while maintaining reasonable computational simplicity.
Solution Approach 2:
The patent replaces the mechanical/volumetric method of determining dilution factor (based on physical volume measurements) with a chemical/concentration-based method. By substituting volume measurement with concentration measurement of a normal component, the system achieves accuracy despite hemolysis.
3Productivity
If multiple target components are analyzed from a small blood sample, then the efficiency of blood utilization is improved, but the measurement accuracy deteriorates due to insufficient sample volume and hemolysis
Solution Approach 1:
The patent applies feedback by measuring a normal component concentration to determine the dilution factor, which then enables accurate calculation of multiple target component concentrations from the same diluted sample. This approach allows efficient use of small blood volumes while maintaining accuracy across multiple analytes.
Solution Approach 2:
The patent creates a universal dilution correction method that can be applied to analyze multiple different target components from a single diluted sample. The dilution factor derived from normal component concentration serves all subsequent measurements, enabling multi-analyte analysis from minimal blood volume.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables high-accuracy analysis of multiple target components even in hemolyzed blood samples by correcting for hemolysis-induced errors, ensuring reliable measurement of concentrations in small blood volumes.
Implementation Method 1
a step of diluting a collected blood sample with a diluent solution
Implementation Method 2
the collected blood is separated into blood cells and blood plasma by a separation membrane
Data Source
AI summary
An object of the present invention is to provide a blood analysis method and a blood test kit, which are capable of performing measurement of a plurality of target components to be analyzed with high accuracies in a case where hemolysis occurs in a case of measuring an amount of target components to be analyzed in a small volume of a blood sample. According to the present invention, provided is a blood analysis method for measuring a concentration of a target component to be analyzed, the blood analysis method including: a step of diluting a collected blood sample with a diluent solution, a step of determining a dilution factor by using a normal value of a normal component which is homeostatically present in blood, a step of analyzing the concentration of the target component to be analyzed in the blood sample, measuring an amount of hemolysis in the blood sample, correcting the dilution factor according to the measured amount of hemolysis, and analyzing the concentration of the target component to be analyzed using the corrected dilution factor.