Blood Analyzer Single Hemolytic Agent White Blood Cell Classification
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Solution Overview
Problem
Current blood analyzers require separate reagents for classifying white blood cells and detecting malaria-infected red blood cells, leading to increased complexity and burden for users.
Innovation Solution
A blood analyzer and method that uses a single hemolytic agent with varying dilution magnifications to classify white blood cells into four groups and detect malaria-infected red blood cells, employing fluorescent and scattered light information, and a staining agent with a fluorescent dye to differentiate malaria-infected cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If separate dedicated reagents are used for classifying white blood cells and detecting malaria-infected red blood cells, then the classification and detection functions can be performed accurately, but the device complexity and user burden increase due to requiring multiple different reagents
Solution Approach 1:
The patent applies universality by designing a single hemolytic agent that serves multiple functions: it hemolyzes red blood cells to enable malaria parasite detection, and simultaneously preserves white blood cell integrity for classification. This multi-functional reagent eliminates the need for separate dedicated reagents for each function, reducing device complexity while maintaining accurate classification and detection capabilities
Solution Approach 2:
The patent merges the functions of separate hemolytic and staining reagents into a single integrated hemolytic agent formulation. By combining the hemolytic components and fluorescent staining components into one reagent system, the patent reduces the number of separate reagents from two or more to one, thereby simplifying the overall system while preserving the ability to perform both white blood cell classification and malaria-infected red blood cell detection
2Reliability
If multiple different reagents are used for different blood cell analysis functions, then each function can be optimized, but the ease of operation deteriorates due to increased user burden in managing multiple reagents
Solution Approach 1:
The single hemolytic agent is designed with universal applicability for multiple blood analysis functions including white blood cell classification and malaria parasite detection. This universal reagent eliminates the need for users to manage, store, and operate multiple different reagents, significantly reducing user burden while maintaining optimized performance for each analysis function through the reagent's multi-functional formulation
3Device complexity
If a single hemolytic agent with varying dilution magnifications is used, then the device complexity and user burden are reduced, but the measurement precision may be affected by the need to optimize different dilution conditions for different analysis purposes
Solution Approach 1:
The patent applies dynamics by implementing a dilution magnification adjustment mechanism that allows the system to dynamically select appropriate dilution levels based on the specific analysis function being performed. The control portion automatically adjusts the dilution magnification of the single hemolytic agent according to whether white blood cell classification or malaria parasite detection is being performed, ensuring optimal measurement precision for each function while maintaining a simple single-reagent system
Solution Approach 2:
The patent utilizes parameter changes by varying the dilution magnification of the single hemolytic agent to optimize performance for different analysis functions. The control portion modifies the concentration parameter of the hemolytic agent through different dilution magnifications, enabling precise control over the reagent's effectiveness for either white blood cell classification or malaria-infected red blood cell detection, thereby maintaining high measurement precision despite using a single reagent
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate classification of white blood cells into multiple groups and detection of malaria-infected red blood cells using a common reagent, reducing the need for multiple reagents and simplifying the analysis process.
Implementation Method 1
a light information generation portion generating first fluorescent information and at least two types of first scattered light information from the first measurement sample and generating second fluorescent information and second scattered light information from the second measurement sample
Data Source
AI summary
This blood analyzer includes a sample preparation portion preparing a first measurement sample containing a blood sample and a hemolytic agent and a second measurement sample containing the blood sample, the same hemolytic agent as the hemolytic agent and a staining agent and a control portion classifying white blood cells in the first measurement sample into at least four groups of monocytes, neutrophils, eosinophils and others on the basis of fluorescent information and two types of scattered light information generated by a light information generation portion and classifying blood cells in the second measurement sample into at least malaria-infected red blood cells and others on the basis of fluorescent information and scattered light information generated by the light information generation portion.