Blood Analyzer Single-Channel Leukocyte Classification via Time-Division Multiplexing
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Solution Overview
Problem
Existing blood analysis methods, such as the single-channel and two-channel methods, face challenges in reliability, sample volume requirements, reagent usage, test speed, and analyzer complexity, necessitating the development of a more accurate and cost-effective blood analysis method.
Innovation Solution
A blood analysis method that involves injecting a blood sample and a first reagent into a reaction cell to obtain a first testing sample for leukocyte classification, followed by the addition of a second reagent for further reaction to obtain a second testing sample for basophil classification or HGB measurement, utilizing a single reaction cell and detection apparatus.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If a single-channel method is used for leukocyte classification, then the analyzer structure is simplified, but the reliability of basophil differentiation is insufficient
Solution Approach 1:
The patent divides the leukocyte classification process into two distinct channels: a DIFF channel for general leukocyte classification and a BASO channel for specialized basophil detection. This segmentation allows each channel to be optimized for its specific function, with the BASO channel using a second reagent specifically designed to differentiate basophils from other leukocytes, thereby resolving the reliability issue while maintaining overall structural efficiency
Solution Approach 2:
The reaction cell is designed to perform multiple functions: it serves as the reaction vessel for both the DIFF channel and BASO channel, and the detection apparatus can detect both general leukocyte classification results and basophil-specific results. This multi-functionality reduces the need for separate dedicated structures, maintaining simplicity while achieving reliable basophil differentiation
2Reliability
If a two-channel method is used for leukocyte classification, then the reliability of basophil differentiation is improved, but the analyzer structure becomes more complex and costs increase
Solution Approach 1:
The patent merges the reaction cell and detection apparatus into shared resources between the DIFF and BASO channels. Instead of having completely separate systems, both channels utilize the same reaction cell for sample preparation and the same detection apparatus for result acquisition, thereby reducing structural complexity and costs while maintaining the reliability benefits of dual-channel classification
3Reliability
If a two-channel method is used for leukocyte classification, then the reliability of basophil differentiation is improved, but the test speed decreases
Solution Approach 1:
The patent implements continuous processing where the DIFF channel and BASO channel operate in parallel using the same sample aliquot. The reaction cell continuously processes samples through both channels simultaneously, and the detection apparatus continuously acquires results from both channels, eliminating sequential processing delays and maintaining high test speed while achieving reliable basophil differentiation
4Adaptability or versatility
If conventional blood analysis methods are used, then the analysis coverage is limited, but the reagent usage and sample volume requirements increase
Solution Approach 1:
The reaction cell and reagent system are designed to perform multiple functions: the first reagent enables general leukocyte classification in the DIFF channel, while the second reagent enables basophil-specific detection in the BASO channel. A single sample aliquot serves both purposes, reducing the total sample volume and reagent requirements compared to separate conventional methods, while expanding analysis coverage to include both general classification and specialized basophil detection
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables efficient leukocyte five-group classification and HGB measurement using a single measurement channel, simplifying the analyzer structure, reducing costs, and improving the price-performance ratio while maintaining accuracy and reliability.
Implementation Method 1
a first reagent is mixed with a sample to obtain a first testing sample for leukocyte classification
Implementation Method 2
a second reagent is mixed with the first testing sample for a further reaction to get a second testing sample for BASO classification and/or HGB measurement
Data Source
AI summary
The present disclosure provided a blood cell analyzer, a control device and a blood analysis method thereof. In the method, a first reagent is mixed with a sample to obtain a first testing sample, and then a second reagent is mixed with the first testing sample for a further reaction to get a second testing sample for basophil classification and/or HGB measurement. A blood sample may be tested in one reaction cell through time-division multiplexing technology to obtain four groups leukocytes classification result and HGB result by single detection channel. Thus, the structure of the analyzer may be greatly simplified on the premise of guaranteeing the performance of the analyzer, the size and cost of the analyzer may reduce and a performance-price ratio of the analyzer may increase.