Blood Analyzer Plasma Measurement to Correct Unknown Interferences
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Solution Overview
Problem
Existing blood gas analyzers struggle to accurately measure hemoglobin forms in the presence of unknown extracellular interferents without requiring field notices, sample studies, verification studies, and new software releases.
Innovation Solution
A blood analyzer that separates plasma from a blood sample, measures the optical signal through both whole blood and plasma samples, and adjusts the total absorbance spectrum using the plasma data to correct for unknown extracellular interferents, eliminating their effects in real time.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional optical measurement is used to determine hemoglobin forms, then measurement process is simple, but measurement precision deteriorates due to unknown extracellular interferents
Solution Approach 1:
The patent segments the blood sample into two distinct components for separate measurement: plasma (extracellular) and whole blood or lysed blood (intracellular). By measuring the plasma sample first to capture interferent signatures, then measuring the whole blood sample and subtracting the plasma contribution, the system isolates and eliminates the effect of unknown extracellular interferents on hemoglobin form measurements without requiring identification of specific interferent types.
Solution Approach 2:
The patent extracts the interferent signal by separately measuring the plasma sample and removing its contribution from the total blood sample measurement. The plasma sample measurement captures the optical absorbance of extracellular interferents, which is then subtracted from the whole blood measurement to isolate the intracellular hemoglobin signal, effectively taking out the interfering component from the measurement.
2Measurement precision
If plasma separation is performed to correct for interferents, then measurement precision improves, but processing time increases
Solution Approach 1:
The patent performs preliminary measurement of the plasma sample before measuring the whole blood sample. By first capturing the interferent signature in the plasma measurement, the system prepares the correction data in advance, allowing rapid subtraction from the subsequent whole blood measurement. This preliminary action eliminates interferents without requiring time-consuming identification or additional correction steps later.
3Reliability
If unknown interferents are present in blood sample, then measurement reliability deteriorates, but device complexity cannot be increased through software updates
Solution Approach 1:
The patent enables the measurement system to self-correct for unknown interferents by automatically performing dual measurements (plasma and whole blood) and computationally subtracting the plasma contribution. The system serves itself by internally identifying and eliminating interferent effects through the measurement protocol, without requiring external software updates, field notices, or manual intervention to maintain reliability when new interferents are encountered.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate determination of hemoglobin forms by effectively removing the influence of unknown extracellular interferents, ensuring precise measurements without the need for additional software updates or sample preparation steps.
Implementation Method 1
The detector obtains an absorbance measurement of the emitted light signal at specific wavelengths following the Beer-Lambert law
Implementation Method 2
A chemical reaction of the sample-reagent combination produces chromophores absorbing light at specific wavelengths proportional to the concentration of the analyte being measured
Data Source
AI summary
Analyzers and methods of use are disclosed, including a blood analyzer comprising a light source to transmit an optical signal; a detector to generate data indicative of optical signal intensity; a transparent sample vessel between the light source and the detector; a dispensing device to pass a first portion of the blood sample comprising whole blood or lysed blood into the vessel at a first instance of time, and to pass a plasma portion of the blood sample into the vessel at a second instance of time; a controller to cause a processor to obtain first and second data generated by the detector, the first data indicative of the optical signal passing through the first portion of the blood sample and the second data indicative of the optical signal passing through the plasma, to determine a total absorbance spectrum in which the first data is adjusted by the second data.