Blood Analyzer Platelet Subpopulation Detection via Optical Scattergram
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Solution Overview
Problem
Current blood analysis methods based on the electrical impedance principle struggle to differentiate between large platelets, red blood cell fragments, and microcytes due to similar volumes, leading to inaccurate counting and increased costs from requiring separate detection channels and reagents for platelet subpopulations.
Innovation Solution
An analysis method and system that uses optical signals, including scattered light and fluorescent signals, to generate scattergrams, allowing for the differentiation of platelet subpopulations like large platelets and immature platelets without the need for separate channels or reagents, by lysing red blood cells and staining white blood cells with fluorescence dyes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If electrical impedance principle is used for blood cell analysis, then the analysis system structure is simple and cost is low, but the measurement precision is insufficient to differentiate particles with same or similar volume
Solution Approach 1:
The patent combines the platelet detection function with the existing white blood cell detection channel by using the same optical detection system. The lytic reagent used for white blood cell detection also enables platelet subpopulation differentiation, merging two detection functions into one channel without requiring separate detection systems.
Solution Approach 2:
The optical detection channel originally designed for white blood cell analysis is made multi-functional by enabling it to also detect and differentiate platelet subpopulations. The same light source, detector, and signal processing system serve both white blood cell classification and platelet subpopulation identification purposes.
2Measurement precision
If separate detection channel and reagents are used for platelet subpopulation differentiation, then the measurement precision is improved, but the device complexity and cost increase
Solution Approach 1:
The patent merges platelet detection with the white blood cell detection channel. The lytic reagent that lyses red blood cells and enables white blood cell detection also allows platelet subpopulations to be differentiated based on their light scattering properties, eliminating the need for a separate detection channel.
Solution Approach 2:
The white blood cell detection channel is given multi-functionality by enabling it to simultaneously perform white blood cell classification and platelet subpopulation differentiation. The same optical system and reagents serve both purposes, reducing device complexity and cost.
3Measurement precision
If separate reagents are used for platelet subpopulation detection, then the measurement precision is improved, but the loss of substance and cost increase
Solution Approach 1:
The patent combines platelet detection reagents with the white blood cell detection reagents. The lytic reagent used for white blood cell analysis also enables platelet subpopulation differentiation, eliminating the need for separate platelet-specific reagents and reducing reagent costs.
Solution Approach 2:
The lytic reagent is made multi-functional by enabling it to simultaneously facilitate white blood cell detection and platelet subpopulation differentiation. This single reagent performs multiple functions, reducing the total amount and cost of reagents required.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate classification and counting of platelet subpopulations, reducing costs and improving analysis accuracy by utilizing existing hemolysis and white blood cell detection channels in blood analyzers, providing detailed information on platelet subpopulations without increasing the cost of the analysis system or reagents.
Implementation Method 1
blood cells in the test sample are stained by a fluorescence dye
Implementation Method 2
the at least two types of optical signals comprise scattered light signals and/or fluorescent signals
Data Source
AI summary
Disclosed are a blood analysis system, a blood analyzer, a blood analysis method and a storage medium. The blood analysis method includes: acquiring at least two types of optical signals of a test sample derived from a blood sample, wherein red blood cells in the test sample are lysed, blood cells in the test sample are stained by a fluorescence dye, and the at least two types of optical signals include scattered light signals and/or fluorescent signals; generating a scattergram based on the at least two types of optical signals; identifying a preset region in the scattergram based on the at least two types of optical signals; and acquiring detection data of a platelet subpopulation based on the preset region.