Blood Analyzing Method for Piroplasm Detection
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Solution Overview
Problem
Current methods for detecting piroplasm-infected red blood cells in dogs and horses are not accurate enough, as they fail to effectively differentiate infected cells from uninfected ones using existing fluorescent dyes and detection techniques.
Innovation Solution
A method involving the use of a blood sample mixed with a fluorescent dye and a cationic surfactant, such as quaternary ammonium salts, to enhance membrane permeability and differentiate infected red blood cells through fluorescence and scattered light information analysis using flow cytometry.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a fluorescent dye is used to stain nucleic acid in red blood cells, then fluorescence information can be obtained for detection, but the ability to accurately differentiate piroplasm-infected red blood cells from uninfected ones is insufficient
Solution Approach 1:
A cationic surfactant is introduced as an intermediary substance that selectively interacts with piroplasm-infected red blood cells. The surfactant binds to the infected cells, creating a distinct complex that enhances fluorescence signal and enables reliable differentiation from uninfected cells through flow cytometry analysis.
Solution Approach 2:
The invention changes the chemical parameters of the staining system by combining fluorescent dyes with cationic surfactants. This parameter change modifies the fluorescence characteristics of infected cells, creating a distinguishable signal pattern that improves detection accuracy and differentiation capability simultaneously.
2Measurement precision
If existing fluorescent staining methods are used, then nucleic acid can be visualized, but the measurement precision for detecting piroplasm-infected red blood cells remains inadequate
Solution Approach 1:
The invention utilizes fluorescence emission (optical color change) by combining specific fluorescent dyes with cationic surfactants. The surfactant-fluorophore complex produces enhanced and distinct fluorescence signals that provide rich information about infected cell characteristics, enabling precise parasitemia measurement without information loss.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for precise detection and counting of piroplasm-infected red blood cells, improving the accuracy of piroplasm infection diagnosis in animals.
Implementation Method 1
a cationic surfactant, such as quaternary ammonium salts, to enhance membrane permeability
Implementation Method 2
a fluorescent dye that stains nucleic acid... acquiring fluorescence information by irradiating with light a measurement sample
Implementation Method 3
acquiring scattered light information... detecting the piroplasm-infected red blood cell in the blood sample based on the fluorescence information and the scattered light information
Data Source
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AI summary
Disclosed is a blood analyzing method comprising: acquiring fluorescence information by irradiating with light a measurement sample prepared by mixing a blood sample collected from a living organism, a fluorescent dye that stains nucleic acid, and a cationic surfactant; and detecting a piroplasm-infected red blood cell in the blood sample based on the fluorescence information.