Blood Cell Analysis Using Multi-Wavelength Scattering and Fluorescence
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Solution Overview
Problem
Existing methods for analyzing blood cells do not effectively account for the influence of white blood cells, which can interfere with the accurate classification and counting of reticulocytes and platelets.
Innovation Solution
A method that utilizes fluorescence information, first scattered light information, and second scattered light information, obtainable by staining blood cells with a fluorescent dye and irradiating them with specific wavelengths of light, to identify and classify blood cells other than white blood cells into reticulocytes and platelets, while suppressing the influence of white blood cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional methods are used to count blood cells, then all blood cells are measured together, but white blood cells interfere with the accurate classification and counting of reticulocytes and platelets
Solution Approach 1:
The patent segments the blood cell analysis by using multiple light wavelengths (first wavelength 315-600nm, second wavelength 610-750nm) to generate different scattered light information and fluorescence information. This segmentation allows white blood cells to be identified and excluded based on their unique scattering patterns at different wavelengths, thereby improving the accuracy of reticulocyte and platelet classification without interference from white blood cells.
Solution Approach 2:
The patent adds dimensional discrimination by measuring scattered light at two different wavelengths and combining it with fluorescence information. This multi-dimensional approach creates a more robust classification space where white blood cells can be distinguished from reticulocytes and platelets based on their unique optical signatures across multiple dimensions (first scattered light, second scattered light, fluorescence), enabling accurate exclusion of white blood cell interference.
2Measurement precision
If multiple parameters are used to classify blood cells, then classification accuracy improves, but the complexity of the analysis system increases
Solution Approach 1:
The patent employs a multi-functional analysis system that simultaneously measures first scattered light, second scattered light, and fluorescence using a single integrated device. This universal system performs multiple functions (white blood cell identification, reticulocyte classification, platelet classification) in one unified platform, avoiding the need for separate devices for each measurement and thereby reducing overall system complexity while maintaining high classification accuracy.
Solution Approach 2:
The patent merges the measurement of first scattered light information, second scattered light information, and fluorescence information into a single integrated analysis process. By combining these multiple parameters in one unified measurement system and processing them simultaneously, the patent achieves accurate blood cell classification without requiring separate complex devices for each parameter, thus balancing measurement precision with system complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate classification and counting of reticulocytes and platelets, while minimizing the impact of white blood cells, thereby improving the reliability of blood cell analysis.
Implementation Method 1
fluorescence information generated by irradiating a measurement sample that contains a particle stained with a fluorescent dye with a light of a first wavelength
Implementation Method 2
first scattered light information, second scattered light information, and fluorescence information generated by irradiating a measurement sample
Data Source
AI summary
A method for analyzing blood cells includes acquiring first scattered light information, second scattered light information, and fluorescence information generated by irradiating a measurement sample that contains a particle stained with a fluorescent dye with a light of a first wavelength and a light of a second wavelength. The method includes identifying blood cells other than white blood cell from the particles in the measurement sample, with reference to the first scattered light information and the second scattered light information, and/or with reference to the first scattered light information and the fluorescence information. The method includes classifying the blood cells other than white blood cell into reticulocyte and platelet, with reference to the fluorescence information and the second scattered light information The first wavelength can be 315 nm or longer and 600 nm or shorter, and the second wavelength can be 610 nm or longer and 750 nm or shorter.