Blood Collection Device with Affinity Molecules for Protein Isolation
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Solution Overview
Problem
Current methods for isolating target components from blood samples are not specific enough, often removing desirable proteins and increasing the risk of contamination and processing errors, as they do not utilize affinity molecules to target specific components during the clean-up process.
Innovation Solution
A device and method for collecting blood that incorporates affinity molecules exposed to the blood during collection, which bind to target components, allowing for specific isolation and reduction of undesired proteins, using a reservoir with immobilized affinity molecules such as antibodies on beads or tubing surfaces, facilitating centrifugation for separation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If current albumin clean-up methods are used to remove high abundance components from blood samples, then the concentration of high abundance proteins is reduced, but the specificity is insufficient and desirable proteins are also removed
Solution Approach 1:
The patent introduces affinity molecules as intermediary substances that specifically bind to target proteins (such as albumin) during blood collection. These affinity molecules act as mediators between the blood sample and the separation process, enabling selective removal of high abundance proteins while preserving desirable low abundance proteins through specific molecular recognition and binding interactions.
Solution Approach 2:
The patent changes the chemical parameter of the blood collection system by incorporating affinity molecules with specific binding properties. This parameter change enables the system to distinguish between different protein types based on their molecular characteristics, achieving selective removal of target proteins while maintaining other proteins in the sample.
2Ease of manufacture
If sample transfer into another container is required for clean-up, then the clean-up process can be performed, but additional handling steps increase the risk of processing errors and sample contamination
Solution Approach 1:
The patent merges the blood collection function with the protein separation function into a single integrated device. The affinity molecules are incorporated directly into the collection tube or container, allowing both sample collection and clean-up to occur in the same vessel without transfer steps, thereby reducing handling errors and contamination risks while maintaining clean-up effectiveness.
Solution Approach 2:
The patent creates a multi-functional blood collection device that simultaneously performs sample collection, protein binding, and separation functions. This universal device eliminates the need for multiple separate操作步骤, reducing the complexity of the workflow and minimizing opportunities for errors and contamination while maintaining all necessary clean-up capabilities.
3Manufacturing precision
If affinity molecules are used to specifically target components during blood collection, then the specificity of isolation is improved, but the device complexity increases
Solution Approach 1:
The patent applies local quality by incorporating affinity molecules only in specific locations within the blood collection device where they are most effective, such as on the inner surface of the collection tube or on magnetic beads. This localized application achieves high specificity for target protein isolation while minimizing the overall complexity of the device by avoiding unnecessary components throughout the entire system.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces handling steps, minimizes contamination risk, and enables accurate analysis by specifically isolating target components during blood collection, improving the separation of low-abundance proteins from high-abundance ones like albumin.
Implementation Method 1
the affinity molecules are exposed to blood during the time of collecting the blood
Implementation Method 2
facilitating centrifugation for separation
Data Source
AI summary
The invention relates to a device and method for collecting blood whereby certain target components are isolated or removed from the blood sample at the time of collecting the blood, as well as methods for using such devices.