Blood Sensor Hemolyzing Agent for Hct Correction
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Solution Overview
Problem
Existing blood component measurement sensors require laborious correction processes to account for hematocrit (Hct) effects, which complicate the measurement of blood components like glucose levels.
Innovation Solution
Incorporating a hemolyzing agent in the sensor's reagent portion to break down erythrocytes, allowing hemoglobin to react with a mediator and generate a current that corrects for Hct effects during redox current detection, eliminating the need for complex correction processes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a correction table or complicated calculation is used to remove Hct effect, then measurement accuracy is improved, but device complexity and operation complexity increase
Solution Approach 1:
The sensor automatically corrects Hct effects through the hemolyzing agent that自发ly breaks down erythrocytes and the electrode that自发ly detects the resulting current, eliminating the need for external correction tables or calculations. The system serves itself by incorporating the correction function directly into the measurement process.
Solution Approach 2:
The invention changes the physical state of erythrocytes from intact to hemolyzed by adding a hemolyzing agent, which releases hemoglobin that then reacts with the mediator to generate detectable current. This parameter change (from intact cells to released hemoglobin) enables automatic Hct compensation without complex processing.
2Extent of automation
If a hemolyzing agent is added to hemolyze erythrocytes and detect hemoglobin reaction current, then Hct effect correction is automated, but reagent portion complexity increases
Solution Approach 1:
The hemolyzing agent serves multiple functions: it breaks down erythrocytes to release hemoglobin, and the released hemoglobin then reacts with the mediator to generate current for Hct correction. This multi-functionality allows a single addition to the reagent portion to achieve both cell lysis and correction signal generation.
Solution Approach 2:
The invention merges the Hct correction function with the blood component measurement function by using the same electrode and mediator system to detect both the blood component reaction current and the hemoglobin reaction current. This consolidation eliminates the need for separate correction mechanisms.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for automatic correction of Hct effects, providing accurate blood component measurements without the need for additional correction processes, thereby simplifying the measurement process.
Implementation Method 1
causing a redox reaction between the blood component and an oxidoreductase in the presence of a mediator; detecting a redox current generated by the redox reaction by electrodes
Implementation Method 2
an erythrocyte is hemolyzed so as to cause hemoglobin released to an outside of the erythrocyte
Implementation Method 3
cause hemoglobin released to an outside of the erythrocyte to react with the mediator and a current generated by this reaction also is detected to correct an effect of a hematocrit
Data Source
AI summary
A sensor for blood component analysis that can correct the effect of a hematocrit easily is provided. The sensor includes an analysis portion including a working electrode, a counter electrode, and a reagent portion. The reagent portion includes an oxidoreductase that reacts with the blood component and a mediator, and the blood component is measured by causing a redox reaction between the blood component and the oxidoreductase in the presence of the mediator and detecting a redox current generated by the redox reaction by the working electrode and the counter electrode. In this sensor, the reagent portion further includes a hemolyzing agent (e.g., sodium cholate) for hemolyzing an erythrocyte, and when detecting the redox current, the erythrocyte is hemolyzed with the hemolyzing agent so as to cause hemoglobin released to an outside of the erythrocyte to react with the mediator and a current generated by this reaction also is detected to correct an effect of a hematocrit.