Multi-Chamber Blood Separation Container for Mononuclear Cell Purity
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional biological sample isolation devices fail to isolate white blood cells effectively for cancer diagnosis, as they often mix white blood cells with a large quantity of specific gravity adjusters, reducing cell bioavailability.
Innovation Solution
A biological sample isolation container with an isolation chamber, collection chamber, waste liquid chamber, and siphon flow paths that utilize centrifugal force and siphoning to separate and collect specific components like mononuclear cells without mixing them with the adjuster, maintaining cell bioavailability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional centrifugal isolation is used to separate white blood cells, then separation is achieved, but white blood cells are mixed with large quantity of specific gravity adjuster reducing cell bioavailability
Solution Approach 1:
The container is divided into multiple functional chambers: isolation chamber for initial centrifugal separation, collection chamber for receiving and re-centrifuging the isolated component, and waste liquid chamber for disposal. This segmentation allows the specific component to be isolated and then re-processed to remove the specific gravity adjuster, thereby improving cell bioavailability while maintaining separation purity
Solution Approach 2:
The patent extracts the specific component (white blood cells) from the mixture of blood and specific gravity adjuster through centrifugal separation. The isolated component is then transferred to the collection chamber where it is re-centrifuged to separate the cells from the specific gravity adjuster, effectively extracting the harmful substance while preserving the valuable cells
2Ease of operation
If manual collection of isolated components is performed, then flexibility is maintained, but labor intensity and potential contamination increase
Solution Approach 1:
The system performs automatic collection through the integrated multi-chamber container design with siphon flow paths that enable automated transfer of liquids between chambers. The container itself provides the collection function through its structured chambers, eliminating the need for manual intervention and reducing contamination risk while maintaining operational ease
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device efficiently isolates and collects mononuclear cells without reducing their bioavailability, ensuring high-quality samples for diagnostic purposes.
Implementation Method 1
The siphon flow path connects the isolation chamber and the collection chamber, and uses siphoning to send the liquid, which contains a specific component that has been isolated for each component by centrifuging of the biological sample and reagent, from the isolation chamber to the collection chamber
Implementation Method 2
biological sample isolation container that is placed in a rotating device and rotates around a specific rotation center to apply centrifugal force, thereby collecting a specific component contained in a biological sample that has been isolated for each component
Implementation Method 3
The collection chamber holds the specific component centrifuged in the isolation chamber, some of the reagent, and a first component contained in the biological sample, which have been sent from the isolation chamber, and after these are suspended, causes only the specific component to settle outward in the radial direction in which centrifugal force is applied
Implementation Method 4
The second siphon flow path connects the collection chamber and the waste liquid chamber, and uses siphoning to send the waste liquid from the collection chamber to the waste liquid chamber, out of the liquid stored in the collection chamber
Data Source
Figure 1
Figure 2
Figure 3
AI summary
A container (30) is provided with an isolation chamber (33), a collection chamber (34), a waste liquid chamber (35), a siphon flow path (33a), and a siphon flow path (34a). The isolation chamber (33) holds a specific gravity adjusting agent (L2) and blood (L1), which have been layered. The collection chamber (34) holds a centrifuged mononuclear cell layer (L4), some of the specific gravity adjusting agent (L2), and plasma (L5), and allows the mononuclear cell layer (L4) to settle in a liquid after being suspended. The waste liquid chamber (35) holds the supernatant liquid of the suspension as waste liquid. The siphon flow path (33a) connects the isolation chamber (33) and the collection chamber (34), and uses siphoning to send a liquid containing the mononuclear cell layer (L4). The siphon flow path (34a) connects the collection chamber (34) and the waste liquid chamber (35), and uses siphoning to send the waste liquid.