Blood Volume Estimation via Image Analysis and Disposable Substrates
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Solution Overview
Problem
Current methods for estimating blood sample volumes on filter paper are inaccurate and costly, especially in non-laboratory settings, due to factors like hematocrit and capillary effects, and existing devices face issues with contamination, organization, and efficient processing of samples.
Innovation Solution
A method involving obtaining an image of the blood sample, calculating the coverage ratio by pixel counting, and comparing it to a standard curve to estimate volume, combined with a device for holding and scanning blood samples that ensures consistent and accurate processing, reducing human error and contamination risks.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional venipuncture method is used for blood collection, then accurate blood volume measurement is achieved, but the procedure becomes more invasive and requires specialized training
Solution Approach 1:
The patent employs disposable filter papers for blood collection that are inexpensive and single-use, eliminating the need for complex reusable equipment and specialized training while maintaining adequate measurement capability through image analysis of the dried blood spots
2Ease of operation
If DBS sampling is used in non-lab settings, then cost and invasiveness are reduced, but accurate quantitation of analytes becomes difficult due to unknown blood volume
Solution Approach 1:
The patent replaces traditional mechanical/measurement-based volume assessment with optical/image-based analysis. By capturing images of dried blood spots and analyzing their visual characteristics, the system estimates blood volume without requiring physical measurement tools or laboratory equipment, enabling accurate quantitation in field settings
Solution Approach 2:
The patent introduces an intermediary estimation approach using visual markers and image analysis as a bridge between simple DBS collection and accurate analyte quantitation. The system uses intermediate steps (image capture, visual analysis, standard curve comparison) to translate qualitative blood spot appearance into quantitative volume and analyte concentration data
3Device complexity
If image-based volume estimation is used, then cost is reduced and ease of operation is improved, but measurement precision may be compromised compared to laboratory methods
Solution Approach 1:
The patent applies preliminary calibration by creating standard curves from known blood volumes before field use. This pre-established reference data allows the image analysis system to accurately estimate unknown volumes by comparison, compensating for the simplicity of the imaging approach and maintaining measurement precision without complex equipment
Data Source
AI summary
Methods and devices for analyzing blood sample volumes are provided. In particular, the disclosure provides a method for estimating the volume of a blood sample on a substrate including the steps of acquiring an image of the blood sample. A coverage or area of the blood sample may be obtained from the image and compared to a standard curve to obtain a volume estimate of the blood sample. The disclosure also proves a device for scanning one or more blood samples on a substrate. The device includes three layers that may be assembled to hold multiple samples between the layers. The device also includes labels and may be disassembled for decontamination and reloading of samples.