Bone Grafts with Stem Cells on Synthetic Scaffolds
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Solution Overview
Problem
Current bone grafts lack the combination of osteogenic, osteoinductive, and osteoconductive properties without the drawbacks of autografts, such as limited availability and donor site morbidity.
Innovation Solution
Development of bone grafts and constructs that incorporate stem cells on a scaffold, combined with osteoinductive demineralized bone and osteoconductive cortico-cancellous chips, to promote bone healing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If autografts are used to provide osteogenic properties, then bone healing is promoted, but donor site morbidity and limited availability occur
Solution Approach 1:
The patent extracts and isolates specific osteogenic stem cells from the autograft material, separating the useful osteogenic component from the harmful donor site morbidity. By taking out only the necessary stem cells and placing them on a scaffold, the invention eliminates the need for donor site surgery while preserving bone healing capability.
Solution Approach 2:
The patent creates a copy of the osteogenic properties by culturing stem cells in vitro and seeding them onto synthetic scaffolds. This copy contains the essential bone-forming capability without requiring actual autograft material, thus avoiding donor site morbidity while maintaining therapeutic effect.
2Reliability
If autografts are used to provide osteogenic properties, then bone healing is promoted, but limited availability occurs
Solution Approach 1:
The patent enables the scaffold to serve itself by incorporating osteogenic stem cells that can autonomously differentiate and form bone. The stem cells are self-sufficient once seeded, requiring no additional autograft material supply, thus eliminating availability limitations while maintaining bone healing promotion.
Solution Approach 2:
The patent changes the state of osteogenic cells from being embedded in autograft material to being cultured as isolated stem cell suspensions. This parameter change allows unlimited replication and standardization of the osteogenic component, overcoming the limited availability of autograft material.
3Object-affected harmful factors
If stem cells are seeded on synthetic scaffold, then osteogenic properties are achieved without donor site morbidity, but the scaffold lacks natural ECM cues
Solution Approach 1:
The patent creates a composite structure combining synthetic scaffold with osteogenic stem cells. The synthetic scaffold provides mechanical strength and stability, while the stem cells provide biological activity and ECM production. This composite achieves both the benefits of synthetic materials and natural tissue integration.
Solution Approach 2:
The patent uses the stem cells as an intermediary between the synthetic scaffold and the host tissue. The stem cells adhere to the synthetic scaffold, produce natural ECM, and differentiate into osteoblasts, effectively bridging the gap between artificial material and biological tissue.
Data Source
AI summary
Bone grafts and constructs including stem cells are provided. Example bone grafts include osteogenic stem cells seeded on a scaffold of osteoconductive cortico-cancellous chips and/or osteoinductive demineralized bone. Example constructs include extracellular matrix on a synthetic scaffold, in which the ECM is secreted from MSCs seeded onto the synthetic scaffold. Also provided are methods of making the present bone grafts and scaffolds. Further provided are methods of promoting bone healing and treating wound healing, by administering the present bone grafts and constructs to a mammal in need thereof. Also provided are kits that include the present bone grafts and/or constructs, or components thereof.