Bone Marrow Mononuclear Cell Sheet for Fibrosis Inhibition
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Solution Overview
Problem
Current fibrosis treatments are limited by the lack of effective therapeutics and associated side effects, and the labor-intensive management of cell sheets in regenerative medicine, which increases costs and hinders the widespread application of regenerative medicine products.
Innovation Solution
A cell sheet comprising bone marrow mononuclear cells, treated with a compound having a Wnt/β-catenin signal inhibitory effect, is created by forming a sheet and subsequent suspension culture, enhancing fibrosis inhibitory action and maintaining high MMP activity for extended periods, allowing for effective treatment of various tissues.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional fibrosis inhibitors are used for treatment, then fibrosis can be addressed, but side effects occur and therapeutic effectiveness is limited
Solution Approach 1:
The patent uses a cell sheet as a disposable therapeutic product that can be applied directly to the target tissue. The cell sheet comprises bone marrow mononuclear cells cultured on a temperature-responsive culture dish, which can be harvested and applied as a single-use therapeutic agent, eliminating the need for long-term drug administration and associated side effects
Solution Approach 2:
The cell sheet performs self-service by utilizing the inherent regenerative and anti-fibrotic capabilities of bone marrow mononuclear cells. The cells naturally secrete factors that inhibit fibrosis and promote tissue repair, eliminating the need for external pharmacological intervention and its associated side effects
2Reliability
If cell sheets are used in regenerative medicine, then therapeutic effects are achieved, but labor-intensive management increases costs
Solution Approach 1:
The cell sheet has multi-functionality, serving both as a delivery vehicle for bone marrow mononuclear cells and as a culture substrate. The temperature-responsive culture dish enables automated sheet formation and release, reducing manual labor for cell sheet preparation and application
Solution Approach 2:
The patent utilizes temperature parameter changes to control cell sheet formation and release. By lowering the temperature of the culture dish, the cell sheet automatically detaches from the culture surface, enabling automated harvesting without manual intervention and reducing labor costs
3Reliability
If multiple cell sheets are stacked to achieve therapeutic effect, then treatment efficacy is improved, but management complexity and costs increase
Solution Approach 1:
The patent merges multiple cell sheets into a single laminated structure. The cell sheets can be stacked and bonded together to form a multi-layered therapeutic product, simplifying the application process while maintaining enhanced therapeutic efficacy through increased cell dosage
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The enhanced fibrosis inhibitory effect of the cell sheet, as measured by increased MMP activity, provides a more effective and cost-efficient therapeutic option for treating fibrosis in regenerative medicine, with improved physical strength and prolonged stability.
Implementation Method 1
A cell sheet is obtained by culturing cells collected from a patient's tissue or an established cell line and making them into a sheet. A certain amount of cells that have been increased by culture are bonded to each other to form a single-layer sheet.
Implementation Method 2
Since adhesion proteins such as the extracellular matrix are maintained at the bottom of the cell sheet, the cell sheet engrafts to the transplant tissue without surgical suturing.
Data Source
Figure 1A~1B
Figure 2~3
AI summary
[Problem to be solved by the invention] To provide a cell sheet for increasing fibrosis inhibitory action, said cell sheet comprising bone marrow mononuclear cells, and a production method thereof. [Solution] A cell sheet comprising bone marrow mononuclear cells, said cell sheet being obtained by forming bone marrow mononuclear cells from a bone marrow mononuclear cell suspension into a sheet, and then shrinking and suspension culturing the result.