Bone Paste Collagen Gelatin Phase Transition
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Solution Overview
Problem
Existing methods for preparing bone paste are complex, inhomogeneous, and require sterile conditions, leading to issues with injectability and potential health risks due to unsterilized osteoinductive proteins, making them costly and limited in practical use.
Innovation Solution
A method involving an initial mixture of an aqueous solution and a granular product with collagen and minerals, heated to at least 100°C to transform collagen into gelatin, creating a cohesive and sterile bone paste with improved rheology and osteoconductive properties, eliminating the need for additional binders and ensuring sterility.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If existing methods prepare bone paste by mixing demineralized bone and water to obtain viscous phase, then bone paste can be formed, but the process becomes complex and the product is inhomogeneous
Solution Approach 1:
The patent combines the viscous phase preparation and solid phase mixing into a single simultaneous heating process. All components (demineralized bone, water, and additional bone material) are heated together at temperatures between 60-121°C, allowing the viscous phase to form in situ and mix homogeneously with the solid phase, eliminating the need for separate preparation steps and ensuring uniform distribution throughout the bone paste.
Solution Approach 2:
The patent utilizes temperature as a critical parameter to control the phase transformation of collagen to gelatin. By heating to specific temperature ranges (60-121°C), the collagen in the bone material transforms into gelatin, creating the viscous phase in situ. This parameter-driven approach simplifies the process by using thermal energy to automatically generate the binding medium rather than requiring separate preparation of viscous phase.
2Reliability
If mixing is carried out in sterile environment, then sterility is ensured, but the process complexity and cost increase
Solution Approach 1:
The patent applies sterilization heating (at temperatures of 121°C or higher) to the complete bone paste mixture after all components are combined. This preliminary sterilization action ensures that the final product is sterile while allowing the mixing process itself to occur under less restrictive conditions. The heat treatment serves dual purposes: completing the collagen-to-gelatin transformation and simultaneously sterilizing the entire mixture.
Solution Approach 2:
The heating step serves multiple functions simultaneously: (1) transforms collagen to gelatin to create viscous phase, (2) mixes and homogenizes the bone paste, and (3) sterilizes the entire mixture. This multi-functional approach eliminates the need for separate sterile mixing equipment and procedures, reducing complexity while ensuring sterility through the universal application of thermal processing to the complete formulation.
3Reliability
If cortical bone is subjected to heat treatment and acid treatment to extract gelatin, then osteoinductive effect is obtained, but the method becomes complex and expensive
Solution Approach 1:
The patent combines heat treatment and acid treatment into a single integrated process step. The bone material is subjected to both thermal processing (60-121°C) and acidic conditions simultaneously, allowing gelatin extraction and collagen transformation to occur together in one operation rather than requiring sequential separate treatments. This reduces process complexity and equipment requirements while maintaining the osteoinductive properties.
Solution Approach 2:
The patent optimizes the temperature parameter range (60-121°C) to achieve collagen-to-gelatin transformation without requiring extreme heat treatments. This moderate temperature range, combined with acidic conditions, efficiently extracts gelatin and creates the viscous phase while preserving osteoinductive proteins, simplifying the overall process compared to more aggressive heat treatment methods.
4Manufacturing precision
If heating is applied to transform collagen into gelatin, then continuity between phases is obtained, but energy consumption increases
Solution Approach 1:
The patent optimizes the heating temperature range to 60-121°C, which is sufficient to transform collagen to gelatin and create phase continuity without requiring excessive energy input. This controlled parameter approach ensures complete collagen denaturation and gelatin formation for homogeneous bone paste while minimizing energy consumption by avoiding unnecessarily high temperatures.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method produces a homogeneous, malleable, and cohesive bone paste that is ready for use, ensuring sterility and safety, with the ability to be directly injected, overcoming the limitations of previous methods by simplifying the process and enhancing the bone paste's properties.
Implementation Method 1
heating step during which the initial mixture is heated in the container at a temperature of at least 100° C., preferably 121° C., to transform a portion of the collagen into gelatin
Implementation Method 2
the heating step enabling sterilization of the bone paste in the container
Data Source
AI summary
The invention relates to a method for preparing an osteoconductive bone paste by heating an initial mixture containing an aqueous solution and a granular material including collagen and minerals. A portion of the collagen and the minerals are contained in grains of bone matrix. The initial mixture is heated enough to transform a portion of the collagen into gelatine and thus to obtain a cohesive bone paste having a predefined viscosity.