Bone Substrate Seeding with Adipose-Derived Stem Cells
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Solution Overview
Problem
Regenerative medicine requires an abundant source of human adult stem cells that can be readily available at the point of care for effective bone formation in tissue engineering, and existing methods for processing adipose-derived stem cells are complex and inefficient.
Innovation Solution
A method involving the digestion of adipose tissue to form a cell suspension containing mesenchymal stem cells and unwanted cells, followed by seeding onto a bone substrate, culturing to allow adherence, and rinsing to remove unwanted cells, resulting in a seeded bone substrate for allograft products.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional methods are used to process adipose-derived stem cells, then stem cells can be obtained for bone formation, but the processing is complex and inefficient
Solution Approach 1:
The processing method is divided into distinct sequential steps: digestion of adipose tissue to release cells, filtration to separate unwanted cells, and seeding onto bone substrate. This segmentation allows each step to be optimized independently and simplifies the overall process control
Solution Approach 2:
Adipose tissue is digested and cells are released before seeding onto the bone substrate. This preliminary preparation ensures that only viable, free-floating stem cells are available for seeding, improving efficiency by preventing the need for complex in-situ processing
2Reliability
If allografts are seeded with living cells, then surgical results are improved, but significant tissue processing and cellular processing is required
Solution Approach 1:
Stem cells are extracted from adipose tissue through enzymatic digestion and filtration, separating the desired cellular component from the unwanted adipose matrix and non-adherent cells. This extraction provides a concentrated source of viable stem cells for seeding, improving surgical outcomes while simplifying the processing required
Solution Approach 2:
The bone substrate itself serves as the selection medium during the seeding process. When the cell suspension is applied, the substrate naturally retains adherent stem cells while allowing unwanted cells to be rinsed away, eliminating the need for complex external processing systems
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method provides a consistent and efficient way to combine mesenchymal stem cells with bone substrates, enhancing bone formation and regeneration by ensuring the adherence of stem cells while removing unwanted cells, thus improving surgical outcomes.
Implementation Method 1
digesting the adipose tissue to form a cell suspension having the mesenchymal stem cells and the unwanted cells
Implementation Method 2
culturing the mesenchymal stem cells on the seeded bone substrate for a period of time to allow the mesenchymal stem cells to adhere to the bone substrate
Data Source
AI summary
There is disclosed a method of combining mesenchymal stem cells (MSCs) with a bone substrate. In an embodiment, the method includes obtaining tissue having MSCs together with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The cell suspension is added to the substrate. The substrate is cultured to allow the MSCs to adhere. The substrate is rinsed to remove unwanted cells. In various embodiments, the tissue is adipose tissue, muscle tissue, or bone marrow tissue. In an embodiment, there is disclosed an allograft product including a combination of MSCs with a bone substrate in which the combination is manufactured by culturing MSCs disposed on the substrate for a period of time to allow the MSCs to adhere to the substrate, and then rinsing the substrate to remove unwanted cells from the substrate. Other embodiments are also disclosed.