Boron-Free Protease Stabilizers for Detergent Enzyme Storage
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Solution Overview
Problem
Existing detergents and cleaning agents face challenges in stabilizing enzymes, particularly proteases, during storage due to autoproteolysis and hydrolysis, leading to activity loss, especially in water-containing formulations, and boron-based stabilizers form undesirable by-products.
Innovation Solution
Incorporating boron-free compounds with a specific structural formula, such as 3-methyl-1,2-benzisothiazole-1,1-dioxide, that act as reversible protease inhibitors, forming non-covalent complexes with proteases to prevent hydrolysis during storage and dissociating during use, ensuring enzyme stability and preventing boron-based contamination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If boron-based stabilizers (e.g., boric acid, phenylboronic acid) are used to stabilize proteases during storage, then enzyme stability is improved, but undesirable by-products are formed during the cleaning process
Solution Approach 1:
The patent extracts and eliminates the boron component from the stabilizer molecule, replacing it with alternative chemical structures (e.g., compounds containing nitrogen and sulfur atoms in heterocyclic rings) that maintain the protease inhibition function during storage but do not form undesirable by-products during the cleaning process
Solution Approach 2:
The patent employs stabilizers with temporary functionality - they are effective during storage (inhibiting protease activity) but are designed to be consumed or deactivated during the cleaning process, allowing the protease to function without inhibition and avoiding persistent by-product formation
2Productivity
If water-containing formulations are used for cleaning agents, then cleaning effectiveness is improved, but enzyme hydrolysis and activity loss increase during storage
Solution Approach 1:
The patent introduces water-soluble stabilizer compounds as intermediary substances that mediate between the aqueous environment and the enzyme. These stabilizers form protective complexes with proteases in the water-containing formulation, preventing hydrolysis during storage while allowing the water to remain available for cleaning effectiveness
Solution Approach 2:
The patent modifies the chemical parameters of the formulation by introducing specific stabilizer compounds with particular molecular structures (e.g., heterocyclic rings with nitrogen and sulfur atoms) that change the local chemical environment around the enzyme, reducing hydrolysis susceptibility while maintaining water solubility and cleaning effectiveness
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The boron-free compounds effectively stabilize proteases and other enzymes during storage, maintaining activity and preventing unwanted by-product formation, with favorable inhibition constants and good water solubility, ensuring efficient cleaning performance without boron-related issues.
Implementation Method 1
forming non-covalent complexes with proteases to prevent hydrolysis during storage
Implementation Method 2
These must be reversible protease inhibitors, as the protease activity should only be temporarily inhibited, particularly during storage
Data Source
AI summary
The present invention relates to detergents and cleaning agents containing at least one protease and at least one organic compound of formula (I) which acts as a protease inhibitor and is thus a suitable enzyme stabilizer, and to the use of said compounds as an enzyme stabilizer in protease-containing detergents and cleaning agents. The invention also relates to the corresponding washing and cleaning methods and to the use of the agents described herein.