Bos Taurus Superovulation Using Single-Dose Peptide-Linked FSH
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Solution Overview
Problem
Current methods for inducing superovulation in Bos taurus cows are time-consuming, stressful, and inefficient, often requiring multiple injections of FSH, which can lead to lower fertilization rates and are not easily extrapolated across different cow subtypes.
Innovation Solution
A single administration of Follicle Stimulating Hormone (FSH) at a dose of 200 μg to 400 μg, preferably 300 μg to 400 μg, is used to induce superovulation and Ovum Pick-Up (OPU) in Bos taurus cows, utilizing a recombinant FSH covalently linked by a peptide linker.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If multiple FSH injections are administered throughout 4 to 5 days, then superovulation is achieved, but the process becomes time-consuming and stressful
Solution Approach 1:
The patent modifies the pharmacokinetic parameters of FSH by creating a long-acting formulation with extended half-life, allowing the hormone to remain active in the body for 24-48 hours. This parameter change enables reduction from multiple daily injections to a single injection while maintaining superovulation efficiency
Solution Approach 2:
The long-acting FSH formulation ensures continuous hormonal action throughout the critical follicular development period. The extended half-life maintains stable FSH levels in the bloodstream, providing uninterrupted stimulation of follicle growth and ovulation without requiring repeated administrations
2Productivity
If multiple FSH injections are administered throughout 4 to 5 days, then superovulation is achieved, but the process becomes stressful for the animal
Solution Approach 1:
The long-acting FSH formulation provides continuous hormonal stimulation throughout the critical period, eliminating the need for repeated injections. This continuous action reduces stress by avoiding multiple handling events while maintaining effective follicular development and ovulation
3Productivity
If standard FSH products with short half-life are used, then superovulation can be induced, but multiple daily treatments are required which are subject to error
Solution Approach 1:
The patent fundamentally changes the half-life parameter of FSH from approximately 6 hours to 24-48 hours through molecular modification. This parameter change creates a more reliable treatment protocol with fewer administration events, reducing the opportunity for errors and improving treatment consistency across different animals and operators
4Productivity
If estrus-based protocols are used, then embryo production is efficient, but the method requires estrus detection and is conditioned by the stage of the estrus cycle
Solution Approach 1:
The long-acting FSH formulation enables preliminary synchronization of follicular waves before the estrus cycle progresses to estrus. By initiating treatment in advance and using the extended half-life to establish uniform follicular development, the protocol creates optimal conditions for superovulation regardless of the exact timing of estrus expression, thereby improving ease of operation while maintaining high embryo production
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method achieves high superovulation rates, producing a significant number of viable embryos and oocytes, improving insemination and pregnancy rates while reducing the stress and time associated with multiple injections.
Implementation Method 1
utilizing a recombinant FSH covalently linked by a peptide linker
Data Source
AI summary
The present invention relates to methods and compositions for increasing superovulation in Bos taurus cows using Follicle Stimulating Hormone.