Bovine Bone Graft Prion Removal via Sodium Hypochlorite and Heat

Solution Overview

Problem

Existing bone graft substitutes, particularly those made from bovine bone, face challenges such as slow tissue regeneration due to structural differences from human bone and the risk of bovine spongiform encephalopathy, which cannot be completely eliminated by existing high-temperature processing methods.

Innovation Solution

A method involving the treatment of bovine bone with sodium hypochlorite to inactivate prion proteins, followed by high-temperature heating at 600°C to create a prion-free bone graft substitute that mimics human bone structure, enhancing tissue regeneration and safety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Shape

If bovine bone is used as a raw material for bone graft substitute, then the structure similar to human bone is obtained, but the risk of bovine spongiform encephalopathy infection remains

Engineering Contradiction:

Improvebone structure similarityVSAvoidprion infection risk

Core Design Contradiction:

ShapeVSObject-affected harmful factors

Solution Approach 1:

The patent applies preliminary action by treating bovine bone with sodium hypochlorite solution before high-temperature heating to inactivate prion proteins. This pre-treatment step ensures that prions are deactivated before the final processing, preventing any potential infection risk while preserving the bone's structural similarity to human bone.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent utilizes parameter changes by heating the sodium hypochlorite-treated bone at high temperatures (600-1000°C) for extended periods (1-6 hours). This extreme thermal treatment fundamentally changes the physical and chemical parameters of the bone material, ensuring complete prion elimination while maintaining the beneficial structural characteristics.

Inventive Principle:

Principle #35Parameter changes

2Object-affected harmful factors

If high temperature treatment at 600°C is applied to remove prion, then prion reduction is achieved, but prion is not completely removed

Engineering Contradiction:

Improveprion contaminationVSAvoidprion-free status

Core Design Contradiction:

Object-affected harmful factorsVSReliability

Solution Approach 1:

The patent applies preliminary action by treating bovine bone with sodium hypochlorite solution before high-temperature heating to inactivate prion proteins. This pre-treatment step ensures that prions are deactivated before the final processing, preventing any potential infection risk while preserving the bone's structural similarity to human bone.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent employs a composite processing approach combining chemical treatment (sodium hypochlorite) with physical treatment (high-temperature heating). This multi-method composite approach ensures complete prion elimination by attacking the prion structure through multiple mechanisms, achieving reliable prion-free status that single methods cannot guarantee.

Inventive Principle:

Principle #40Composite materials

3Ease of manufacture

If commercial bone substitutes are used, then availability and homogeneity are improved, but tissue regeneration rate is slow due to structural differences

Engineering Contradiction:

Improveavailability and homogeneityVSAvoidtissue regeneration rate

Core Design Contradiction:

Ease of manufactureVSProductivity

Solution Approach 1:

The patent utilizes parameter changes by heating the sodium hypochlorite-treated bone at high temperatures (600-1000°C) for extended periods (1-6 hours). This extreme thermal treatment fundamentally changes the physical and chemical parameters of the bone material, ensuring complete prion elimination while maintaining the beneficial structural characteristics.

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method produces a bone graft substitute with improved osteoconductivity and safety by effectively removing lipids and proteins, reducing the risk of bovine spongiform encephalopathy and promoting faster bone regeneration.

Implementation Method 1

treating bovine bone with sodium hypochlorite to inactivate prion protein

Methodology Applied

Scientific EffectOxidation: Oxidation

Implementation Method 2

heating the treated bone at a high temperature of more than 600° C.

Methodology Applied

Scientific EffectThermal decomposition: Pyrolysis

Data Source

PatentUS8586099B2Method for preparing a prion-free bond grafting substitute

Publication Date: 2013.11.19 SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION

AI summary

The present invention relates to a method for preparing a bone graft substitute using bovine bone, and more particularly to a method for preparing a safe bone graft substitute which does not have the risk of infection with bovine spongiform encephalopathy, the method comprising treating bovine bone with sodium hypochlorite and treating the treated bone at a high temperature of more than 600° C. The bone graft substitute does not cause an immune response, because it is prepared by effectively removing lipids and organic substances from bovine bone having a structure very similar to that of the human bone. Also, it has excellent osteoconductivity, and is free of prion, and thus it does not have the risk of infection with bovine spongiform encephalopathy. According to the disclosed invention, the bone graft substitute having such advantages can be prepared in a simple manner.