Extending Bovine Cell Replicative Capacity via TERT and Proliferation Modulation
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
The mass production of cell-based meat products from cultured metazoan cells is limited by senescence, leading to increased costs and resource requirements, as existing methods fail to adequately increase the replicative capacity of bovine cells necessary for large-scale production.
Innovation Solution
Introducing a polynucleotide encoding telomere reverse transcriptase (TERT) and at least one polynucleotide encoding a protein capable of modulating cell proliferation into bovine cells, using non-integrating methods to enhance replicative capacity and reduce doubling time, allowing for the production of sufficient cells for cell-based meat products.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If TERT alone is introduced into bovine fibroblast cells, then replicative capacity increases in other species, but replicative capacity does not increase in bovine cells
Solution Approach 1:
The patent combines TERT introduction with the introduction of at least one additional polynucleotide encoding a protein capable of modulating cell proliferation. This merging of multiple genetic modifications resolves the contradiction by recognizing that TERT alone is insufficient in bovine cells, but the combination of TERT with other proliferation-modulating proteins achieves the desired replicative capacity extension.
Solution Approach 2:
The patent changes the genetic parameter by introducing multiple polynucleotides rather than just TERT. This parameter change (from single-gene to multi-gene introduction) adapts the approach to bovine cell specificity, overcoming the limitation where TERT alone fails to increase replicative capacity in this species.
2Productivity
If conventional cell culture methods are used, then cells can be maintained, but senescence limits mass production capability
Solution Approach 1:
The patent performs preliminary genetic modification by introducing TERT and other proliferation-modulating polynucleotides into bovine cells before mass production begins. This preliminary action extends the cellular lifespan and prevents senescence during the production process, enabling sustained mass production capability.
Solution Approach 2:
By introducing multiple polynucleotides that modulate cell proliferation, the patent ensures continuous cell division and prevents the natural cessation of cell growth associated with senescence. This maintains the useful action of cell production over extended periods, enabling sustained mass production.
3Quantity of substance
If more starting cells are used to overcome senescence, then adequate cell numbers can be achieved, but costs and resource requirements increase
Solution Approach 1:
The patent changes the biological parameters of the cells by introducing TERT and proliferation-modulating proteins, which increases the intrinsic replicative capacity and reduces doubling time. This parameter change allows achieving adequate cell numbers with fewer starting cells and reduced resource consumption compared to conventional methods.
Solution Approach 2:
The patent enhances the copying capability of cells by introducing TERT, which maintains telomere length and enables repeated cell division. This improved copying capacity allows cell populations to be expanded more efficiently from smaller starting numbers, reducing the resources needed for mass production.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly increases the population doubling level of bovine cells, reducing the number of starting cells needed and maintaining replicative capacity after multiple doublings, thereby facilitating the production of cell-based meat products with improved efficiency and cost-effectiveness.
Implementation Method 1
introducing into a cell of the population of cells a polynucleotide encoding telomere reverse transcriptase (TERT)
Implementation Method 2
introducing into the cell of step (b) at least a first polynucleotide encoding a first protein that is capable of modulating cell proliferation
Data Source
AI summary
Provided herein are methods of method for extending the replicative capacity of a bovine cell. For example, a method for extending the replicative capacity of a bovine cell includes (a) isolating a population of cells from bovine tissue; (b) introducing into a cell of the population of cells a polynucleotide encoding telomere reverse transcriptase (TERT); (c) introducing into the cell of step (b) at least a first polynucleotide encoding a first protein that is capable of modulating cell proliferation; and (d) culturing the population of bovine cells in a cultivation infrastructure is provided herein.