Bovine Hemoderivative Production via Inert Atmosphere and Segmented Extraction
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Solution Overview
Problem
Existing methods for producing bovine hemoderivatives face issues such as energy loss, chemical instability due to oxidizing atmospheres, low selectivity in etheric preparation, and microbial contamination, leading to incomplete removal of undesirable substances and potential explosions during ether evaporation.
Innovation Solution
A biotechnological method involving multi-phase fermentation, controlled drying, and ethanol extraction with Soxhlet extraction, followed by vacuum inspissation and repeated etheric preparation to achieve high concentration and separation of bovine hemoderivative, using a nitrogen atmosphere to prevent oxidation and microbial contamination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If ethanol extraction is performed with the extract placed outside the boiler, then the extraction process can proceed, but energy is lost and the temperature of the extract reduces
Solution Approach 1:
The patent combines the extraction process with the heating system by placing the extraction vessel inside the boiler, allowing the extract to be heated during extraction. This merges two previously separate operations (extraction and heating) into one integrated process, eliminating energy loss and temperature reduction issues.
2Device complexity
If one-step etheric preparation is used to separate phospholipids, then the process is simpler, but the selectivity is low and undesirable substances remain in the precipitate
Solution Approach 1:
The patent divides the etheric preparation into multiple sequential steps: first etheric extraction to remove phospholipids, then a second etheric treatment of the precipitate to further purify it. This segmentation of the preparation process into distinct stages achieves high purification selectivity while maintaining reasonable process complexity.
3Loss of substance
If ether evaporation is performed to remove ether after etheric preparation, then ether can be removed from the precipitate, but there is risk of explosion and ether contamination of the environment
Solution Approach 1:
The patent performs ether evaporation in an inert atmosphere (nitrogen or carbon dioxide) to eliminate oxygen, thereby preventing explosion risks. The inert gas environment also prevents ether contamination of the surrounding atmosphere, addressing both safety and environmental concerns while effectively removing ether from the precipitate.
4Quantity of substance
If the evaporation residue is left after ether removal, then the concentrated extract is obtained, but it becomes susceptible to microbial contamination and oxidative cleavage
Solution Approach 1:
The patent maintains the evaporation residue in an inert atmosphere (nitrogen or carbon dioxide) to prevent both microbial contamination and oxidative cleavage. The inert gas creates a protective environment that preserves the chemical stability of the concentrated extract while preventing microbial growth, ensuring long-term reliability of the product.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method results in a high-quality, microbially and chemically stable product with minimal undesirable phospholipids and blood salts, ensuring efficient separation and stability, reducing process time and environmental risks.
Implementation Method 1
using a nitrogen atmosphere to prevent oxidation and microbial contamination
Implementation Method 2
The extract of the blood fermentations product is subjected to one vacuum inspissation, usually to a final concentration 20 times higher then the original concentration
Implementation Method 3
the condensed blood fermentation product is subjected to precipitation through diethyl ether, and the obtained precipitate is separated from the solution of undesirable phospholipids dissolved in the ether
Implementation Method 4
ethanol extraction of the blood fermentation product in several phases
Data Source
AI summary
The method of biotechnological production of bovine hemoderivative is presented in the following overview providing a summary of individual technological operations in time sequence (Flow-Sheet).