Bovine Placenta Exosome Extraction via Freeze-Thaw and Ultrasonic Treatment
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Solution Overview
Problem
Current methods for obtaining placenta extracts are inefficient, resulting in low absorption rates, complex product compositions, and low extraction efficiency, which hinders the development of effective cosmetic products.
Innovation Solution
A method for preparing exosomes from bovine placenta using physical extraction techniques such as freeze-thaw, ultrasonic treatment, and filtration, followed by lyophilization to produce a stable lyophilized powder.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If traditional extraction methods (direct extraction, supercritical CO2 extraction, enzyme-assisted extraction) are used to obtain placenta extract, then the extraction process is simple and extensive, but the extraction efficiency and utilization rate of raw materials are low
Solution Approach 1:
The extraction process is divided into multiple sequential steps: homogenization, freeze-thaw cycles, ultrasonic treatment, centrifugation, and filtration. Each step segments the extraction process to progressively isolate exosomes from placental tissue, improving overall extraction efficiency while maintaining operational simplicity
Solution Approach 2:
The placental tissue undergoes preliminary homogenization and freeze-thaw treatment before the actual extraction. These preliminary actions prepare the tissue structure to facilitate more efficient exosome release and separation in subsequent steps, thereby improving utilization rate of raw materials
2Ease of manufacture
If traditional extraction methods are used to obtain placenta extract, then the extraction process is simple, but the obtained extract contains complex components and low quality
Solution Approach 1:
The method specifically extracts exosomes from placental tissue through a series of separation steps including centrifugation at different speeds and filtration through membranes with specific pore sizes. This selective extraction isolates exosomes from other complex placental components, achieving high product purity while maintaining a relatively simple overall process
Solution Approach 2:
Different stages of the extraction process use different conditions (temperature, centrifugal force, filtration pore size) optimized for specific separation tasks. For example, low-speed centrifugation removes large debris while high-speed centrifugation isolates exosomes, and filtration uses specific pore sizes to ensure purity. This localized optimization of extraction conditions achieves high manufacturing precision
3Ease of manufacture
If traditional extraction methods are used to obtain placenta extract, then the extraction process is simple and extensive, but the absorption efficiency of the human body is low
Solution Approach 1:
The method replaces traditional chemical extraction methods with physical mechanisms including ultrasonic vibration, centrifugal force, and filtration. This mechanical approach preserves the integrity and biological activity of exosomes, ensuring they maintain their natural structure for effective human body absorption while keeping the extraction process simple
4Reliability
If exosomes are extracted from bovine placenta using physical extraction techniques, then the extraction rate of exosomes is high with good integrity and biological activity, but the process requires multiple steps including freeze-thaw, ultrasonic treatment, and filtration
Solution Approach 1:
The extraction process employs continuous useful actions where each step builds upon the previous one: homogenization breaks down tissue, freeze-thaw cycles release exosomes, ultrasonic treatment further disperses them, and centrifugation/filtration separates them. This continuous sequence of actions maintains exosome integrity throughout the process while systematically progressing toward the final product
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves a high extraction rate of exosomes with good integrity and biological activity, enabling effective skin repair and anti-aging benefits while maintaining stability and convenience for long-term storage and use.
Implementation Method 1
subjecting the homogenate to freeze-thaw
Implementation Method 2
performing a first round of ultrasonic treatment on the thawed homogenate
Implementation Method 3
thawing the filtrate at 4° C. and centrifuging to obtain a supernatant
Implementation Method 4
performing multiple filtration through filters to obtain the exosomes from bovine placenta
Implementation Method 5
followed by lyophilization to produce a stable lyophilized powder
Data Source
AI summary
Disclosed are a method for preparing exosomes from bovine placenta, lyophilized powder of the exosomes, as well as a composition containing exosomes from animal placenta and a preparation method and use thereof in anti-wrinkle on skin.