Bragg Mirror Light Source for Dark-Field Microscopy

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Solution Overview

Problem

Dark-field microscopy requires complex and expensive equipment for reliable analysis of biological specimens, limiting its application due to the need for specialized objectives and condensers, and existing plasmonic-based methods are cumbersome and require elaborate data analysis.

Innovation Solution

A compact and inexpensive dark field microscopy light source is developed using a Bragg mirror with a light-receiving and light-transmitting surface, in conjunction with a reflector, to create a high-angle illumination cone within the sample, enabling dark-field imaging with standard microscopes through a luminescent micro-patterned photonic substrate with controlled angular emission.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If dark-field microscopy is implemented using traditional specialized equipment (objectives, condensers), then reliable analysis of biological specimens is achieved, but device complexity and cost increase significantly

Engineering Contradiction:
Improveanalysis reliabilityVSAvoidequipment complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent extracts the dark-field illumination function from the complex traditional optical system (objectives, condensers) and implements it through a simplified substrate-based approach. The substrate incorporates a photonic crystal layer that generates high-angle scattered light directly at the sample plane, eliminating the need for specialized dark-field optical components while maintaining reliable biological specimen analysis capability

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent introduces a photonic crystal layer as an intermediary between the light source and the sample. This photonic crystal structure acts as a mediator that transforms conventional light into high-angle scattered light, enabling dark-field illumination without requiring complex optical pathways or specialized microscopy components

Inventive Principle:
Principle #24Intermediary (Mediator)

2Illumination intensity

If conventional dark-field microscopy equipment is used, then high image contrast for small features is achieved, but the system becomes bulky and expensive

Engineering Contradiction:
Improveimage contrastVSAvoidsystem size
Core Design Contradiction:
Illumination intensityVSWeight of stationary object

Solution Approach 1:

The patent creates a functional copy of the dark-field illumination effect using a photonic crystal substrate rather than replicating the entire traditional dark-field optical system. The photonic crystal layer reproduces the high-angle light scattering function that produces dark-field contrast, but in a miniaturized form factor that can be integrated into standard microscope slides or substrates

Inventive Principle:
Principle #26Copying

Solution Approach 2:

The patent changes the physical parameters of light interaction by using a photonic crystal structure with specific lattice constants and refractive index contrasts. This structure modifies the light propagation characteristics to generate high-angle scattered light, achieving dark-field illumination conditions without the bulky optical components traditionally required

Inventive Principle:
Principle #35Parameter changes

3Reliability

If specialized dark-field objectives and condensers are employed, then reliable biological specimen analysis is achieved, but cost and system complexity increase

Engineering Contradiction:
Improvespecimen analysis reliabilityVSAvoidsystem cost
Core Design Contradiction:
ReliabilityVSEase of manufacture

Solution Approach 1:

The patent creates a universal substrate that can be used with standard microscopes to achieve dark-field imaging capability. The photonic crystal substrate serves multiple functions: it generates high-angle scattered light for dark-field illumination, can be fabricated using standard semiconductor manufacturing techniques, and is compatible with conventional microscope optics, thereby reducing system cost and complexity while maintaining analysis reliability

Inventive Principle:
Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This solution simplifies and miniaturizes dark-field microscopy, providing high-contrast images of small organisms without the need for specialized equipment, improving signal-to-noise ratio for weakly scattering materials and enabling imaging of specimens with low refractive index contrast.

Implementation Method 1

a Bragg mirror having a light-receiving surface and a light-transmitting surface for directing light at an object

Methodology Applied
Scientific EffectBragg reflection: Bragg Diffraction

Implementation Method 2

a reflector having a reflective surface facing the light-receiving surface of the Bragg mirror

Methodology Applied
Scientific EffectLight reflection: Reflection

Data Source

PatentUS12164097B2Bragg light source for dark-field imaging devices
Publication Date: 2024.12.10 MASSACHUSETTS INST OF TECH
  • US12164097B2 patent drawing
  • US12164097B2 patent drawing
  • US12164097B2 patent drawing

AI summary

Articles and systems for dark microscopy and related methods are generally described.