Animal Brain Extract Preparation for Neuroprotection
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Solution Overview
Problem
Current methods for preparing extracts from biological materials for the prevention and treatment of neurodegenerative diseases are either ineffective or have significant side effects, and there is a need for a process that can be easily implemented on an industrial scale to produce specific combinations of neurological components.
Innovation Solution
A process involving the extraction of minced brains using a mixture of ethanol:water or chloroform:methanol, followed by distillation and precipitation with acetone, and saponification to obtain a brain extract rich in complex polar lipids, which is suitable for neuroprotection and memory preservation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current methods for preparing extracts from biological materials are used, then extraction of neurological components is achieved, but the methods are either ineffective or have significant side effects
Solution Approach 1:
The patent changes the chemical parameters of the extraction system by using specific solvent ratios (ethanol:water or chloroform:methanol), controlling pH levels, and adjusting temperature conditions to optimize extraction effectiveness while minimizing harmful side effects. This resolves the contradiction by finding optimal parameter ranges that improve reliability without increasing harm.
Solution Approach 2:
The patent introduces specific intermediaries including buffer solutions, precipitating agents, and purification materials that mediate between the raw biological material and the final extract. These intermediaries enable effective extraction of neurological components while filtering out harmful substances, thus improving reliability while reducing side effects.
2Productivity
If a process is designed for industrial scale implementation, then productivity is improved, but manufacturing complexity may increase
Solution Approach 1:
The patent divides the extraction process into distinct sequential stages: initial extraction with solvent mixture, filtration, precipitation, centrifugation, and final purification. Each stage can be independently optimized and scaled for industrial production, improving productivity while keeping individual process steps relatively simple and manageable.
Solution Approach 2:
The patent employs universal equipment and reagents that can be used across multiple extraction steps and are suitable for both laboratory and industrial scale operations. The standardized protocol using common solvents and equipment reduces manufacturing complexity while enabling high productivity through scalable implementation.
3Manufacturing precision
If specific combinations of neurological components are obtained, then product purity is improved, but the extraction process becomes more complex
Solution Approach 1:
The patent selectively extracts specific neurological components from the biological material by using solvents that preferentially dissolve target compounds while leaving other substances behind. The precipitation step further separates specific components by controlling solubility conditions, achieving high manufacturing precision through selective extraction and phase separation.
Solution Approach 2:
The patent creates different local conditions within the extraction system - using pH gradients, temperature zones, and solvent concentration variations to selectively precipitate or dissolve specific neurological components. This local differentiation of extraction conditions enables high specificity without requiring overly complex overall process design.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process yields a brain extract that effectively prevents deficits in working and long-term memory, reduces lipid peroxidation, and has neuroprotective activity, making it suitable for treating neurodegenerative diseases with minimal side effects.
Implementation Method 1
subjecting minced brains to an extraction with a mixture of ethanol:water or with a mixture of chloroform:methanol
Implementation Method 2
distilling the solvent of the liquid fraction L1
Implementation Method 3
precipitating with acetone to obtain the solid fraction, referred to as S2
Implementation Method 4
saponifying the solid fraction S2
Data Source
AI summary
The present invention refers to a process for preparing an animal brain extract. It also refers to the brain extract obtainable according to said process and to the use thereof as a medicine, in particular for the prevention and/or treatment of neurodegenerative diseases and disorders of the central nervous system. It also refers to compositions comprising this extract and to its use for preparing these compositions.