Broken Lactic Acid Bacteria Cells for Lipid Metabolism
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Solution Overview
Problem
Current methods for improving lipid metabolism using lactic acid bacteria are inefficient, with inconsistent results and limited effectiveness, particularly in reducing cholesterol and visceral fat levels, and there is a need for more potent agents to treat lifestyle-related diseases and metabolic syndromes.
Innovation Solution
The use of broken cells of lactic acid bacteria, specifically strains like Lactobacillus amylovorus and Lactobacillus gasseri, which are broken through physical, chemical, or enzymatic means to enhance lipid metabolism by lowering total cholesterol, LDL-cholesterol, and visceral fat while elevating HDL-cholesterol and adiponectin levels.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If intact lactic acid bacteria are used to improve lipid metabolism, then the bacteria can be administered as live probiotics, but the effectiveness is very low and results are inconsistent
Solution Approach 1:
The lactic acid bacteria are broken into smaller fragments through physical disruption methods (ultrasonic treatment, high-pressure homogenization, or mechanical grinding). This segmentation of the bacterial cells releases intracellular active substances while maintaining the overall bacterial structure, thereby enhancing the lipid metabolism improvement effect without requiring complete cell lysis.
Solution Approach 2:
The patent changes the physical state parameter of the lactic acid bacteria from intact whole cells to partially disrupted cells with altered cell wall integrity. This parameter change increases the bioavailability of intracellular components while preserving enough structural integrity to maintain stability during storage and administration.
2Reliability
If organic-solvent extract of lactic acid bacterium is used to provide more potent effect, then the effectiveness is enhanced, but the effect is still limited and unsatisfactory
Solution Approach 1:
The patent selectively extracts specific intracellular components (such as peptidoglycan, lipoteichoic acid, or other cell wall components) from the lactic acid bacteria using targeted extraction methods. This takes out only the most effective components needed for lipid metabolism improvement, avoiding the complexity of complete organic solvent extraction while maintaining high effectiveness.
3Reliability
If large amount of lactic acid bacterium is administered to achieve effectiveness, then the dosage increases, but the effectiveness is still insufficient and placebo controls are inadequate
Solution Approach 1:
The patent concentrates the most effective intracellular components of the lactic acid bacteria into specific fragments or extracts with enhanced local activity. Instead of administering large quantities of whole bacteria, the disrupted cells deliver concentrated active substances directly to the target sites, thereby achieving better effectiveness with smaller dosages.
Data Source
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AI summary
This invention provides an effective means or method for improving lipid metabolism, and a means or method for treatment or prevention of diseases or disorders associated with the lipid metabolism disorder. Specifically, this invention relates to an agent for improving the lipid metabolism comprising, as active ingredients, broken cells of a lactic acid bacterium, and to a method for enhancing an effect of a lactic acid bacterium for improving the lipid metabolism comprising a step of breaking the lactic acid bacterium.