Brucella Broth Preservation for Helicobacter Pylori Resistance Detection

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Solution Overview

Problem

Current methods for detecting antibiotic-resistant Helicobacter pylori genotypes, such as culture-based antimicrobial susceptibility testing and molecular testing, are time-consuming and inefficient, leading to low eradication rates of Helicobacter pylori infections.

Innovation Solution

A method for preserving test samples using brucella broth, combined with primer sets targeting 23S rRNA and gyrA genes for PCR-based detection, and a reagent kit for accurately identifying antibiotic-resistant genotypes, followed by tailored treatment regimens.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If culture-based antimicrobial susceptibility testing is used to guide Helicobacter pylori eradication therapy, then treatment efficacy is improved, but testing time and operational complexity increase significantly

Engineering Contradiction:

Improvetreatment efficacyVSAvoidtesting time

Core Design Contradiction:

ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces the traditional culture-based mechanical testing system with a molecular biology-based detection system. By using PCR amplification and hybridization probes to detect antibiotic resistance genes directly from clinical samples, the method eliminates the need for time-consuming bacterial culture while maintaining accurate resistance detection, thereby resolving the contradiction between treatment efficacy and testing time

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent creates molecular copies (DNA amplification) of the antibiotic resistance genetic markers instead of relying on phenotypic expression through bacterial culture. This copying approach allows direct detection of resistance genes from clinical samples, significantly reducing testing time while preserving the reliability needed for guiding effective eradication therapy

Inventive Principle:

Principle #26Copying

2Productivity

If molecular testing for gene mutations is used to guide Helicobacter pylori treatment, then testing speed is improved, but eradication rate remains insufficient

Engineering Contradiction:

Improvetesting speedVSAvoideradication rate

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent segments the detection process into multiple targeted components: detecting 23S rRNA mutations for clarithromycin resistance, gyrA mutations for levofloxacin resistance, and amoxicillin resistance genes. This segmented multi-gene detection approach maintains fast molecular testing speed while improving eradication rates by providing comprehensive resistance profiling to guide optimal antibiotic selection

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent changes the detection parameters from single-gene or limited-gene testing to a comprehensive multi-parameter panel covering multiple antibiotic resistance mechanisms. By simultaneously detecting mutations in 23S rRNA, gyrA, and amoxicillin resistance genes, the method maintains molecular testing speed while significantly improving treatment reliability through more complete resistance characterization

Inventive Principle:

Principle #35Parameter changes

3Ease of operation

If empirical therapy is used for Helicobacter pylori eradication, then treatment simplicity is improved, but eradication rate decreases due to antibiotic resistance

Engineering Contradiction:

Improvetreatment simplicityVSAvoideradication rate

Core Design Contradiction:

Ease of operationVSReliability

Solution Approach 1:

The patent performs preliminary molecular detection of antibiotic resistance genes before initiating eradication therapy. By pre-identifying which antibiotics the Helicobacter pylori strain is resistant to through rapid molecular testing, the system enables clinicians to select effective antibiotics in advance, maintaining treatment simplicity while overcoming the limitations of empirical therapy caused by antibiotic resistance

Inventive Principle:

Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Improves the efficiency and accuracy of detecting and treating antibiotic-resistant Helicobacter pylori, enhancing eradication rates beyond existing methods.

Implementation Method 1

preserving the test sample in a brucella broth

Methodology Applied

Scientific EffectPreservation: Preservative

Implementation Method 2

performing a polymerase chain reaction on the test sample using the primer set of the reagent kit to obtain a product

Methodology Applied

Scientific EffectPolymerase chain reaction:

Data Source

PatentUS20250327137A1Reagents and methods for preservation and detection of gastric helicobacter pylori

Publication Date: 2025.10.23 NAT TAIWAN UNIV HOSPITAL

AI summary

The present disclosure provides methods for effectively preserving test samples utilized in detecting Helicobacter pylori. The present disclosure also provides methods for accurately detecting antibiotic-resistant genotypes of Helicobacter pylori, as well as methods for treating Helicobacter pylori infected-diseases. The disclosed methods effectively improve the efficiency and practicality of both detecting and treating antibiotic-resistant Helicobacter pylori.