Biosensor Reagent Layer BSA Hematocrit Stability
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional biosensors face challenges in obtaining accurate results due to the influence of hematocrit in blood samples and ambient temperature fluctuations, leading to potential errors in glucose concentration measurements.
Innovation Solution
Incorporating bovine serum albumin (BSA) as a solubilized protein in the reagent layer with enzymes like glucose dehydrogenase having flavin adenine dinucleotide or pyrroloquinoline quinone as coenzymes, which improves the biosensor's resistance to hematocrit and temperature effects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional enzyme sensors are used for glucose measurement in blood samples, then the sensor can perform quantitative analysis of glucose concentration, but the measurement precision deteriorates due to the influence of hematocrit and temperature variations
Solution Approach 1:
The patent introduces bovine serum albumin (BSA) as an intermediary substance in the reagent layer that mediates between the enzyme system and the blood sample components. BSA interacts with hematocrit and temperature variations to stabilize the sensor response, reducing their harmful effects on measurement precision while maintaining the glucose detection function
Solution Approach 2:
The patent modifies the composition parameters of the reagent layer by adding BSA at specific concentrations (0.001-0.1% w/v). This parameter change alters the physical and chemical environment in the reagent layer, making it less sensitive to hematocrit and temperature variations, thereby improving measurement accuracy under varying conditions
2Reliability
If the reagent layer contains only enzyme and electron carrier, then the device complexity is low, but the reliability deteriorates due to sensitivity to hematocrit and temperature
Solution Approach 1:
BSA serves as a stabilizing intermediary in the reagent layer that buffers the effects of hematocrit and temperature without requiring complex additional components. This single additive enhances reliability while maintaining relatively simple device structure
Solution Approach 2:
The reagent layer is formulated as a composite material system containing enzyme, electron carrier, and BSA. This composite structure combines the functional properties of each component, creating a more reliable system that tolerates hematocrit and temperature variations better than simple enzyme-based systems
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The addition of BSA in the reagent layer enhances the biosensor's precision by reducing the impact of hematocrit and temperature on sensor response characteristics, ensuring accurate glucose concentration measurements across varying hematocrit levels and temperatures.
Implementation Method 1
an enzyme which reacts with a specific component in a sample solution
Implementation Method 2
an enzyme sensor for glucose... reduces an electron acceptor by an electron generated by a reaction between an enzyme and a substrate
Implementation Method 3
supply of the sample solution from the cavity 7 into the biosensor is realized by a capillary phenomenon
Data Source
Figure 1~2
Figure 3~4
Figure 5
AI summary
In a biosensor of the present invention, BSA is contained in a reagent layer including a reagent which reacts specifically with glucose, and the glucose concentration in whole blood as a sample solution is electrochemically measured. When the sensor response characteristics when whole blood is used as the sample solution are measured, the influence by hematocrit to the sensor response characteristics is reduced according to the additive amount of BSA when the hematocrit value is in the range of 25% and below, while no BSA addition effect is recognized in the sensor response characteristics when the hematocrit value is in the range of 25 to 65%. Thereby, the influences by hematocrit and temperature can be reduced, and thus highly precise measurement can be performed.