BSH Hapten Conjugate for Sensitive Monoclonal Antibody Detection
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Solution Overview
Problem
Current methods lack a sensitive and selective means for detecting and quantifying mercaptoundecahydrododecaborate (BSH) in vivo, particularly due to its low molecular weight and easy ionization, which hinders effective immunological measurement in boron-neutron capture therapy (BNCT).
Innovation Solution
A hapten compound with a linker bound to the SH group of BSH is used to induce a specific immune response, producing a monoclonal antibody that can detect BSH highly sensitively and selectively, along with a hybridoma and measurement kit for immunological quantification.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a small-molecular inorganic compound like BSH is used as an antigen, then the molecular weight is low and ionization is easy, but the antigenicity is poor making sensitive detection impossible
Solution Approach 1:
The patent applies composite material principle by creating a conjugate of BSH (small-molecular inorganic compound) with a carrier protein (high-molecular compound). This composite structure combines the detection target properties of BSH with the high antigenicity of the carrier protein, enabling sensitive immunological detection while maintaining the ability to specifically recognize BSH
Solution Approach 2:
The patent uses a carrier protein as an intermediary substance that bridges the gap between the low-antigenicity BSH and the immune system. The carrier protein acts as a mediator that enhances antigenicity without interfering with the specific recognition of BSH by the generated antibodies
2Loss of information
If conventional boron-containing compounds are synthesized for BNCT, then therapeutic application is achieved, but in vivo behavior and microscopic distribution remain unknown
Solution Approach 1:
The patent replaces complex physical or chemical analysis methods with an immunological measurement method using specifically generated antibodies. This substitution enables straightforward detection of BSH distribution and concentration in biological samples, providing in vivo behavior information without requiring complex analytical equipment or procedures
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The monoclonal antibody provides excellent sensitivity and specificity for BSH detection, enabling precise and convenient measurement of BSH concentrations, facilitating improved therapeutic outcomes in BNCT by elucidating its microscopic distribution.
Implementation Method 1
an antibody against mercaptoundecahydrododecaborate (BSH), which is obtained by using a conjugate of the above compound as a hapten and a high-molecular compound
Data Source
AI summary
The present invention is a compound having a structure represented by the following formula (1):and an antibody to BSH obtained by using the compound as a hapten and using a complex of the hapten and a high-molecular compound as an antigen. By using the present invention, it becomes possible to provide a hapten compound for preparing an antibody recognizing BSH highly sensitively and highly selectively, an antibody to BSH, as well as a kit for measuring BSH and an immunological measuring method having high sensitivity and excellent in quantitative property using the antibody.