Bst-2 Gene Knockout Cell Line for High-Yield Virus Production
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Solution Overview
Problem
Current methods for producing influenza vaccines face inefficiencies due to the limitations of using fertilized eggs, including security concerns, allergic reactions, and difficulties in expanding production facilities, as well as the need for high initial investments and lower yields per unit volume in animal cell culture methods.
Innovation Solution
Development of a virus-producing mutant cell line with a lack of Bst-2 gene function, achieved through mutations or silencing of the Bst-2 gene, which enhances virus production by reducing apoptosis and promoting extracellular virus release.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If fertilized eggs are used for virus production, then virus yield is obtained, but production efficiency is low and expansion of production facilities is difficult
Solution Approach 1:
The invention extracts and eliminates the limiting factor (Bst-2 gene function) that prevents efficient virus production and release, thereby resolving the contradiction between virus yield and production efficiency without requiring expansion of physical facilities
Solution Approach 2:
The invention changes the genetic parameter of the host cell by mutating or silencing the Bst-2 gene, which fundamentally alters the virus production characteristics and enables high-yield virus production with easier facility expansion
2Productivity
If animal cell culture method is used, then production scale can be expanded, but initial investment is high and yield per unit volume is low
Solution Approach 1:
The invention changes the genetic parameter of animal cells by introducing Bst-2 gene mutations, transforming them into high-yield virus production factories that simultaneously achieve large-scale production capability and high yield per unit volume
Solution Approach 2:
The mutated animal cell line serves multiple functions: it can be cultured at various scales from laboratory to industrial level, produces high virus yield per unit volume, and maintains the scalability advantage of animal cell culture systems
3Reliability
If Bst-2 gene function is present in host cells, then cell defense mechanism is maintained, but virus production and release are inhibited
Solution Approach 1:
The invention extracts and removes the Bst-2 gene function from host cells through mutation or silencing, eliminating the barrier to virus production while maintaining overall cell viability and function
Solution Approach 2:
The invention converts the harmful effect of Bst-2-mediated virus retention into a benefit by creating cell lines where the modified Bst-2 function actually promotes efficient virus production and release, turning a defensive mechanism into a productive feature
Data Source
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AI summary
The present invention relates to a cell line having an increased ability to produce virus and a method for preparing thereof, and more particularly, to a cell line that has an increased ability to produce virus, due to the loss of the function of the Bst-2 (tetherin) gene, and to a production method thereof. According to the present invention, a cell line lacking the function of the Bst-2 gene has an excellent ability to produce virus, compared to a wild-type cell line. Thus, when the mutant cell line is used as a virus-producing cell line, the production yield of virus can be increased. In addition, the mutant cell line can be used for the production and research of vaccines for preventing and treating viral diseases.