Automated Buffer Preparation Control System

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Solution Overview

Problem

Existing methods and tools are inadequate for preparing complex buffer solutions, particularly those involving additional components and salts, and do not support the calculation and control of parameters such as buffer concentration, salt concentration, and flow rates in chromatography systems.

Innovation Solution

A computer-implemented method and tool for calculating and automating the preparation of liquid mixtures, including buffers with multiple buffering species and additives, by determining relative component proportions and pump settings based on desired pH, ionic strength, and buffer capacity, using algorithms and feedback control to ensure precise composition.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional buffer mixing devices are used, then simple buffer solutions can be prepared, but complex buffer solutions with multiple components and salts cannot be properly controlled

Engineering Contradiction:
Improvecapability to prepare complex buffer solutionsVSAvoidcomplexity of control system
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The control system segments the buffer preparation process into multiple independent control loops, each managing a specific parameter (pH, ionic strength, concentration, flow rates). This allows complex buffers with multiple components to be prepared by controlling each aspect separately through dedicated sensors and actuators, thereby achieving versatility without overwhelming complexity.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The system dynamically adjusts control parameters based on real-time feedback from sensors. The control system can adapt flow rates, pump speeds, and mixing conditions on-the-fly to achieve desired buffer composition, enabling preparation of complex buffers with precise control over multiple simultaneous parameters.

Inventive Principle:
Principle #15Dynamics

2Measurement precision

If feedback control is applied to maintain buffer accuracy, then pH control is improved, but control of multiple parameters simultaneously becomes difficult

Engineering Contradiction:
Improveaccuracy of buffer compositionVSAvoidnumber of control parameters
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The feedback control system is segmented into multiple independent control loops, each dedicated to a specific parameter (pH, ionic strength, concentration). Each loop uses its own sensor and actuator pair, allowing precise control of individual parameters without interference from others, thus maintaining accuracy while managing complexity.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The system implements feedback control by continuously monitoring parameters with sensors and adjusting process variables accordingly. Real-time feedback from pH sensors, conductivity sensors, and flow meters enables the control system to maintain precise composition by making dynamic corrections to pump rates and mixing conditions.

Inventive Principle:
Principle #23Feedback

3Adaptability or versatility

If multiple components and salts are added to buffers, then buffer functionality is enhanced, but calculation and control of relative concentrations becomes complex

Engineering Contradiction:
Improvebuffer functionalityVSAvoidease of calculation and control
Core Design Contradiction:
Adaptability or versatilityVSEase of manufacture

Solution Approach 1:

The control system performs self-calibration and automatic calculation of required component concentrations based on target buffer specifications. It automatically determines the precise amounts of each component and salt needed, eliminating manual calculation errors and simplifying the manufacturing process while maintaining enhanced buffer functionality.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The system manages complexity by dynamically adjusting and optimizing parameter relationships. It calculates and controls the relative concentrations of multiple components and salts by changing process parameters (flow rates, mixing ratios) in a coordinated manner, making the preparation of functionally enhanced buffers straightforward despite the increased number of components.

Inventive Principle:
Principle #35Parameter changes

4Productivity

If automated preparation is implemented, then preparation time is reduced, but system complexity increases

Engineering Contradiction:
Improvepreparation speedVSAvoidautomation system complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The automated system performs self-programming and self-execution of buffer preparation protocols. It automatically retrieves component information, calculates required amounts, controls pump operations, and monitors completion without human intervention. This self-service automation reduces preparation time while managing complexity through standardized, pre-configured control logic.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The system replaces manual mechanical operations with automated electronic control. Pump controllers, valve actuators, and sensors work together to automatically measure and mix components, eliminating manual pouring and measurement. This substitution increases productivity by reducing preparation time while managing complexity through software-based control rather than physical complexity.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentEP3931559B1Control of a buffer preparation process
Publication Date: 2026.04.01 CYTIVA SWEDEN AB
  • EP3931559B1 patent drawingFigure 1
  • EP3931559B1 patent drawingFigure 2
  • EP3931559B1 patent drawingFigure 3A

AI summary

The present invention relates to a computer-implemented method for controlling preparation of a liquid mixture comprising an acid and its conjugate base or a base and its conjugate acid. The method comprises obtaining (S11) a composition for the liquid mixture to be prepared and determining (S12) a plurality of settings for controlling a preparation of the liquid mixture in one or more automated processes. The plurality of settings are provided (S13) to one or more control systems of the one or more automated processes. The present invention also relates to a buffer tool configured to perform the computer-implemented method, a buffer management system comprising the buffer tool, and a computer readable storage medium (25), having stored thereon a computer program which, when executed in a buffer tool, causes execution of the computer-implemented method.