Buoyant Particle Cell Therapy Separation System
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Solution Overview
Problem
Conventional cell therapy manufacturing systems face challenges such as overstimulation, low yields, long processing times, cell death, and scalability issues due to magnetic-based separation methods, which hinder the widespread adoption and efficiency of cell therapy.
Innovation Solution
The implementation of a buoyant-particle-assisted cell therapy system that utilizes buoyant particles to separate and process cells, allowing for efficient scaling, minimizing overstimulation, and increasing cell yields by using gravity-based separation mechanisms, which separates cells from co-stimulation factors and prevents phagocytosis, thereby enhancing the cell therapy process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If magnetic-based separation is used for cell therapy manufacturing, then cell separation can be achieved, but cell overstimulation occurs and yields are low
Solution Approach 1:
The patent replaces magnetic-based separation with acoustic-based separation using standing wave fields. This substitution eliminates the need for magnetic beads that cause cell overstimulation, while achieving effective cell separation through acoustic radiation forces that sort cells based on their acoustic impedance without harmful mechanical or magnetic interactions.
Solution Approach 2:
The patent introduces an acoustic field as an intermediary mechanism for cell separation. Instead of direct magnetic interaction with labeled cells, the acoustic standing wave creates a force field that indirectly separates cells based on their physical properties, avoiding the harmful effects of magnetic bead attachment and subsequent overstimulation.
2Productivity
If magnetic-based separation is used for cell therapy manufacturing, then cell processing can be performed, but scalability is limited due to increasing costs for larger magnets
Solution Approach 1:
The patent replaces the scaling-problematic magnetic system with an acoustic field generation system. Acoustic standing waves can be generated in containers of various sizes using transducers, allowing scalable cell processing from small to large volumes without the exponential cost increase associated with larger magnets. The acoustic system scales more linearly with processing volume.
3Productivity
If conventional cell therapy systems are used, then cell processing can be performed, but processing time is long
Solution Approach 1:
The patent enables continuous cell separation through acoustic standing wave fields that can process cells in real-time as they flow through the system. Unlike batch magnetic separation methods that require multiple steps and washing cycles, the acoustic system continuously sorts cells based on their acoustic properties, significantly reducing processing time while maintaining separation efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables efficient scaling, reduces processing time, minimizes cell overstimulation, and increases cell yields, making cell therapy more viable and efficient by using buoyant particles to separate and process cells effectively.
Implementation Method 1
buoyant-particle-assisted cell therapy system that utilizes buoyant particles to separate and process cells, allowing for efficient scaling, minimizing overstimulation, and increasing cell yields by using gravity-based separation mechanisms
Data Source
AI summary
A system for buoyant-particle-assisted cell therapy includes and/or interfaces with a set of buoyant particles. Additionally or alternatively, the system can include and/or interface with a processing container, a set of processing materials (e.g., buffers, factors, solutions, etc.), and/or any other components. A method for buoyant-particle-assisted cell therapy includes processing the set of cells of interest. Additionally or alternatively, the method can include any or all of: preparing a set of buoyant particles; receiving a sample; and isolating a set of cells of interest from the sample.