BVP10 Protein Miticidal Control of Tetranychid Mites
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Solution Overview
Problem
Current methods for controlling tetranychid mites, such as chemical pesticides, lead to resistance issues and economic losses due to the lack of effective biological control options, particularly proteinaceous agents.
Innovation Solution
A method involving the use of a BVP10 protein, encoded by SEQ ID NO.2, is developed for controlling tetranychid mites, which is prepared through prokaryotic expression and shown to have miticidal activity, offering a high-efficiency, low-toxicity, and environmentally friendly biological control option.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chemical pesticides are used to control tetranychid mites, then mite population is reduced, but resistance develops and economic losses increase
Solution Approach 1:
The patent changes the chemical composition parameter from conventional chemical pesticides to a specific protein-based biopesticide (BVP10 from Bacillus velezensis). This parameter change maintains pest control effectiveness while avoiding the resistance development issue that plagues chemical pesticides, thereby resolving the contradiction between maintaining productivity and ensuring reliability of pest control
Solution Approach 2:
The patent employs a protein-based biopesticide that can be rapidly degraded and replaced, unlike persistent chemical pesticides. This approach allows for frequent application of fresh, effective formulations without accumulating resistance, thus maintaining both productivity and reliability over time
2Object-affected harmful factors
If proteinaceous agents are developed for biological control, then environmental friendliness and low toxicity are achieved, but effectiveness against mites must be proven
Solution Approach 1:
The patent utilizes the natural properties of the BVP10 protein produced by Bacillus velezensis, which self-targets mites through specific binding to mite gut receptors. This self-service mechanism ensures high miticidal activity while maintaining environmental safety, as the protein does not require modification to achieve its function
Solution Approach 2:
The patent changes the molecular structure parameters of the biopesticide to match the specific epitope recognition requirements of mite gut receptors. By optimizing the protein's amino acid sequence and three-dimensional structure, the patent achieves both high miticidal activity and environmental friendliness simultaneously
Data Source
Figure 1
AI summary
The invention belongs to the technical field of agricultural microbiology, and in particular, relates to a BVP10 protein shown in SEQ ID NO.2 for controlling tetranychid mites, and use thereof. The BVP10 protein has a median lethal concentration of 19.07 µg/mL against Tetranychus urticae, a median lethal concentration of 58.05 µg/mL against Panonychus citri, a median lethal concentration of 36.08 µg/mL against Tetranychus cinnabarinus, and a control effect of 79.53%-95.45% against strawberry red spider mites.