Downy Mildew Resistant Cabbage Breeding via Marker-Assisted Selection

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Solution Overview

Problem

Cabbage varieties resistant to downy mildew, caused by Hyaloperonospora brassicae, have not been developed effectively due to the lack of useful genetic resources and the difficulty in breeding due to morphological differences with related species like broccoli, leading to high labor and cost costs for chemical fungicide application.

Innovation Solution

Development of markers linked to downy mildew resistance in broccoli, allowing for hybridization with cabbage and subsequent backcrossing to introduce the resistance gene, resulting in a cabbage line with high commercial value and marked resistance to downy mildew.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Object-affected harmful factors

If chemical fungicides are used to control downy mildew, then disease occurrence is reduced, but labor cost and application cost increase

Engineering Contradiction:
Improvedowny mildew occurrenceVSAvoidlabor cost and application cost
Core Design Contradiction:
Object-affected harmful factorsVSEase of manufacture

Solution Approach 1:

The patent develops cabbage varieties with inherent downy mildew resistance through genetic breeding, enabling the crop to protect itself against disease without requiring external chemical interventions. This self-service mechanism eliminates the need for repeated fungicide applications and reduces labor costs associated with disease management

Inventive Principle:
Principle #25Self-service

2Reliability

If broccoli is used as breeding material to introduce downy mildew resistance, then resistance is achieved, but morphological differences make breeding difficult

Engineering Contradiction:
Improvedowny mildew resistanceVSAvoidbreeding complexity due to morphological differences
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent extracts only the essential downy mildew resistance gene from broccoli through molecular marker-assisted backcrossing, while eliminating unwanted morphological characteristics. This selective extraction allows introduction of resistance without importing broccoli's undesirable traits into cabbage

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent uses molecular markers as intermediaries to track and select for the downy mildew resistance gene during the backcrossing process. These markers serve as mediators that enable precise selection of resistant plants while maintaining cabbage morphology, bridging the gap between broccoli's resistance and cabbage's breeding requirements

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS11427832B2Downy mildew resistant cabbage and breeding method therefor
Publication Date: 2022.08.30 SAKATA SEED CORP
  • US11427832B2 patent drawing
  • US11427832B2 patent drawing
  • US11427832B2 patent drawing

AI summary

The present application discloses a cabbage having resistance against downy mildew or its progeny. The present application further discloses a method for breeding downy mildew resistant cabbage, including introducing downy mildew resistance from a Brassica oleracea plant having resistance against downy mildew into desired cabbage. One embodiment of the present invention provides a novel cabbage line showing high resistance against downy mildew and having a high commercial value as cabbage, and enables breeding such cabbage.