Calcium D-glycerate Crystallization via pH and Temperature Control
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Solution Overview
Problem
Current methods for producing glyceric acid and glycerate are inefficient and lack effective approaches for achieving stable crystalline forms, which are crucial for their applications in human metabolism and health benefits.
Innovation Solution
The development of crystalline and amorphous forms of calcium D-glycerate, characterized by specific X-ray powder diffraction patterns, through methods involving mixing D-glyceric acid with calcium salts, adjusting pH, and using solvents, along with a purification process using magnetic nano adsorbents to remove impurities from fermentation broths.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional methods are used to produce glyceric acid and glycerate, then production can proceed with standard processes, but the production efficiency is low and stable crystalline forms cannot be achieved
Solution Approach 1:
The patent applies parameter changes by systematically varying pH values (adjusting to specific ranges like pH 2-4), temperatures (cooling rates of 0.5-5°C/min, aging temperatures of 4-25°C), and solvent compositions (adding organic solvents like ethanol or isopropanol) to transform the production process. These parameter modifications enable the formation of stable crystalline forms of calcium glycerate that were not achievable with conventional standard processes, thereby resolving the contradiction between production efficiency and crystalline stability.
2Ease of manufacture
If fermentation broth is used directly without purification, then the production process is simpler, but impurities such as proteins, pigments, and polysaccharides contaminate the final product
Solution Approach 1:
The patent segments the purification process into distinct sequential stages: first adjusting pH to precipitate proteins and polysaccharides, then filtering to remove solid impurities, followed by adding organic solvents to precipitate remaining contaminants, and finally crystallizing the pure calcium glycerate. This segmented approach maintains relative simplicity while achieving high purity (95% or higher) by addressing different types of impurities in separate steps rather than using a single complex purification method.
3Speed
If rapid cooling is applied to crystallize calcium glycerate, then crystallization speed increases, but the resulting crystals have poor stability and morphology
Solution Approach 1:
The patent implements periodic action through a two-stage temperature control process: first, rapid cooling (0.5-5°C/min) is applied initially to induce nucleation and achieve fast crystallization, then the system transitions to a slower cooling phase or maintains constant low temperature (4-25°C) for extended aging periods (1-24 hours). This periodic temperature adjustment allows the system to benefit from both rapid crystal formation and subsequent stabilization, producing crystals with both good yield and stable morphology characterized by specific XRPD patterns.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The methods produce stable and purified forms of calcium glycerate with improved thermal stability and metabolic benefits, enhancing the efficiency of glyceric acid production and its applications in promoting alcohol metabolism.
Implementation Method 1
mixing D-glyceric acid with a calcium salt to form a first solution
Implementation Method 2
cooling the first solution to a temperature at a cooling rate; aging the first solution for a time period at the temperature
Implementation Method 3
purification process using magnetic nano adsorbents to remove impurities from fermentation broths
Data Source
AI summary
The present disclosure provides a crystalline form of calcium D-glycerate of formulaand a method for preparing the crystalline form of calcium D-glycerate of formula (I). The crystalline form may be characterized by having X-ray powder diffraction pattern (XRPD) diffraction peaks (2θ degrees) at 12.9°±0.2°, 20.9°±0.2°, and 31.7°±0.2°.