Camellia sinensis Stem Cell Extract Ethanol Solvation
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Solution Overview
Problem
Current methods for producing Camellia sinensis stem cell extracts are inefficient in providing effective protection against environmental damage and inflammation, particularly when it comes to UV and drying effects on skin cells, and often rely on synthetic components that consumers prefer to avoid.
Innovation Solution
A process involving dedifferentiated stem cell culture extracts of Camellia sinensis is developed, using ethanol as the extraction solvent to produce a concentrate rich in flavanones, polyphenols, and terpenoids, which are then incorporated into topical or oral compositions to enhance skin resilience and reduce inflammation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional extraction methods (water, methanol, acetone) are used to produce Camellia sinensis stem cell extract, then the extraction process is simple and familiar, but the protective effect against UV and drying damage is insufficient
Solution Approach 1:
The patent changes the extraction solvent from conventional options (water, methanol, acetone) to ethanol, which has different chemical properties that enable more effective extraction of protective compounds from dedifferentiated stem cells. This parameter change in the extraction process yields an extract with superior protective effects against UV and drying damage while maintaining a relatively simple extraction procedure
2Quantity of substance
If dedifferentiated stem cell culture extracts are produced using ethanol extraction, then the concentration of actives (flavanones, polyphenols, terpenoids) is higher and protective effect is improved, but the extraction process becomes more specific and less conventional
Solution Approach 1:
The patent employs ethanol as the extraction solvent, changing the chemical parameter of the extraction system to achieve higher concentrations of bioactive compounds including flavanones, polyphenols, and terpenoids. This single parameter change in the extraction process delivers superior protective effects without requiring complex multi-step procedures or specialized equipment
Solution Approach 2:
The patent uses dedifferentiated stem cells as the starting material, which have been prepared in advance through cell culture processes. This preliminary preparation of the biological material enhances the yield and potency of the extract, allowing ethanol extraction to produce highly concentrated actives efficiently
3Reliability
If conventional stem cell extracts are used, then the production process is established and familiar, but the anti-inflammatory effect and skin protection are noticeably inferior
Solution Approach 1:
The patent changes the extraction solvent parameter to ethanol and uses dedifferentiated stem cells instead of conventional stem cell sources. This combination of parameter changes produces an extract with significantly enhanced anti-inflammatory effects and skin protection while maintaining a straightforward extraction process that is easy to manufacture
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The Camellia sinensis dedifferentiated stem cell extract effectively protects skin cells from UV and drying damage, offering a strong anti-inflammatory effect and improved skin health, with ethanol extraction yielding superior results compared to other solvents, and providing a natural alternative to synthetic compositions.
Implementation Method 1
performing an extraction on the cell culture using ethanol as the extraction solvent, to produce the Camellia sinensis dedifferentiated cell culture extract
Data Source
AI summary
A process for the production of a Camellia sinensis dedifferentiated stem cell extract, the process comprising the steps of: (a) Preparing a cell culture comprising Camellia sinensis dedifferentiated stem cells; (b) Performing an extraction on the cell culture using ethanol and/or methanol as an extraction solvent, to produce the Camellia sinensis dedifferentiated stem cell extract.