Campylobacter Immunogenic Compositions for Poultry Colonization Control

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Solution Overview

Problem

Current methods lack effective interventions to reduce Campylobacter levels in poultry, leading to excessive contamination and potential fines for processing plants, with no available vaccines or intervention methods for producers to control Campylobacter in poultry destined for processing.

Innovation Solution

Development of immunogenic compositions comprising expression vectors encoding proteins or antigenic portions of Cj0998c, Cj0588, and Cj0248 proteins, which are potent immunogens for stimulating an immune response against Campylobacter jejuni, administered to reduce intestinal colonization and infection in subjects such as chickens and humans.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If no vaccine or intervention method is available, then producers cannot control Campylobacter levels in poultry, but this leads to excessive contamination and potential fines for processing plants

Engineering Contradiction:

ImproveCampylobacter control capabilityVSAvoidCampylobacter contamination level

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The vaccine is administered to poultry before they reach processing plants, proactively establishing immune protection in advance. This preliminary immunization prevents Campylobacter colonization before it can cause contamination issues at processing facilities, thereby resolving the contradiction between lacking control capability and experiencing excessive contamination.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The vaccine acts as an intermediary substance that mediates between the poultry host and Campylobacter bacteria. By introducing this immunogenic composition, producers gain indirect control over Campylobacter levels through the activated immune system of the poultry, rather than having direct control methods available.

Inventive Principle:

Principle #24Intermediary (Mediator)

2Quantity of substance

If antibiotics are used to control Campylobacter, then bacterial load may be reduced temporarily, but antibiotic resistance emerges and regulatory restrictions increase

Engineering Contradiction:

ImproveCampylobacter bacterial loadVSAvoidAntibiotic resistance

Core Design Contradiction:

Quantity of substanceVSObject-affected harmful factors

Solution Approach 1:

Instead of using antibiotics that create harmful resistance, the invention converts the harmful presence of Campylobacter into a beneficial immunization opportunity. The bacteria itself (or its antigens) serves as the vaccine stimulus, transforming the pathogen from a purely harmful agent into a tool for creating protective immunity without generating resistance.

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The invention replaces the mechanical/chemical approach of antibiotic killing with a biological approach of immune system activation. Rather than using antibiotics to mechanically reduce bacterial load, the vaccine harnesses the poultry's immune system to naturally control and eliminate Campylobacter, avoiding the creation of resistant strains.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

3Reliability

If vaccination is implemented, then Campylobacter colonization is reduced, but this requires development and implementation of new immunogenic compositions

Engineering Contradiction:

ImproveCampylobacter colonization reductionVSAvoidVaccine composition complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The vaccine composition is segmented into distinct functional components: expression vectors containing specific polynucleotides (Cj0998c, Cj0588, Cj0248), promoters for gene expression, and optional adjuvants. This segmentation allows for systematic development and optimization of each component while maintaining overall vaccine effectiveness, managing the complexity through modular design.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The expression vectors are designed to be universally applicable across different poultry species and Campylobacter strains. The selected antigens (Cj0998c, Cj0588, Cj0248) represent conserved bacterial components that provide broad-spectrum protection, making the vaccine composition multi-functional and reducing the need for strain-specific formulations.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The immunogenic compositions significantly reduce Campylobacter jejuni colonization in poultry and humans by eliciting an effective immune response, providing a potential solution to the high levels of contamination and associated health risks.

Implementation Method 1

potent immunogens for stimulating an immune response against Campylobacter jejuni

Methodology Applied

Scientific EffectImmune response:

Data Source

PatentUS11203618B2Campylobacter immunogenic compositions and uses thereof

Publication Date: 2021.12.21 THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIV OF ARIZONA

AI summary

The present disclosure provides immunogenic compositions against Campylobacter and methods for using the immunogenic composition to generate an immune response against Campylobacter and/or reduce intestinal colonization by Campylobacter.