Candida Strain for Acidified Soil pH Recovery and Phosphorus Release
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Solution Overview
Problem
The limited strain resources and poor adaptability of microorganisms to acidified soil hinder effective remediation, leading to soil acidification, increased heavy metal activity, and reduced nutrient absorption.
Innovation Solution
A strain of Candida XHZG06-95A3, capable of increasing ambient pH and dissolving P components, is used in a microbial fermentation process with mussel shell powder to enhance soil pH and soluble phosphorus content.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Temperature
If chemical improvement agents such as lime are used to alleviate soil acidification, then soil pH can be raised, but soil hardening and fertility decline occur
Solution Approach 1:
The patent converts the harmful effect of chemical improvement agents (soil hardening and fertility decline) into a benefit by using microbial agents that naturally neutralize acidity through metabolic processes. The microbial strain produces alkaline substances and amine compounds that raise pH without causing the adverse effects of chemical lime, thus transforming a harmful approach into a beneficial one.
Solution Approach 2:
The patent introduces a microbial strain as an intermediary between the acidic soil and the desired pH correction. This microbial agent acts as a mediator that naturally neutralizes acidity through biological processes, avoiding the direct harmful impact of chemical lime while achieving the same pH improvement effect.
2Object-affected harmful factors
If microbial improvement agents are used to alleviate soil acidification, then adverse effects on soil are reduced, but strain resources are limited and adaptability to acidified soil is poor
Solution Approach 1:
The patent modifies the parameters of the microbial strain through selective breeding and laboratory evolution to enhance its adaptability to acidified soil conditions. The strain is engineered to tolerate low pH environments and maintain high metabolic activity and pH-neutralizing capability across a wide pH range (3.0-8.0), transforming a strain with poor adaptability into one with excellent versatility.
Solution Approach 2:
The patent creates multiple strains through laboratory evolution and selective breeding, copying and refining the beneficial traits of early microbial agents. This results in a family of strains (including XHZG06-95A3 and its derivatives) that inherit and enhance the adaptability and effectiveness of the original microbial improvement agents.
3Ease of manufacture
If microbial strains with poor stress resistance are used, then strain development is easier, but remediation effect on acidified soil is limited
Solution Approach 1:
The patent performs preliminary selection and laboratory evolution of microbial strains before field application. Strains are pre-adapted to acidic conditions through controlled laboratory exposure and selective breeding, ensuring they possess the necessary stress resistance and remediation capability before being deployed to acidified soil, thus avoiding the need to develop strains from scratch with poor stress resistance.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The strain effectively reduces soil acidity and increases available phosphorus, improving soil fertility and nutrient absorption in acidified conditions.
Implementation Method 1
producing amine substances to neutralize hydrogen ions in the environment
Implementation Method 2
dissolving P components, which can transform insoluble phosphorus into soluble phosphorus
Implementation Method 3
using acidic substances in the environment to reduce the acidity of the environment
Data Source
AI summary
The present invention relates to the field of microorganisms, and discloses a strain of Candida and application thereof. The strain of Candida has a name of XHZG06-95A3, belongs to Candida palmioleophila in taxonomy, and has a preservation number of CGMCC No. 29215. The C. XHZG06-95A3 provided by the present invention has the capacity of increasing ambient pH and dissolving P components, can reduce the acidity of acidified soil and increase the available phosphorus content therein, and can adapt to a wide range of ambient temperatures and pH values. This is the first time that a strain capable of increasing ambient pH and dissolving P components has been found in the species of C. palmioleophila.