Canine EIC Genetic Detection via DNM1 Allele Assay
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Solution Overview
Problem
Current methods lack an effective and reliable way to diagnose Exercise Induced Collapse (EIC) in dogs, particularly Labrador Retrievers, as existing tests are not standardized and often rely on unreliable exercise trials, and there is a need for a genetic marker to predict predisposition to the condition.
Innovation Solution
Development of assays and kits that detect the dynamin 1 (DNM1) G767T allele in canine nucleic acid samples using hybridization, sequencing, or amplification techniques to determine the presence of the biomarker associated with EIC, allowing for genetic screening and predisposition testing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If exercise trials are used to diagnose EIC, then clinical symptoms can be observed, but the diagnosis is unreliable and lacks standardization
Solution Approach 1:
The patent replaces the mechanical/physical exercise trial system with a molecular biological detection system. Instead of observing clinical symptoms during exercise, the invention uses PCR amplification and sequencing of the DNM1 gene to detect the G767T mutation, transforming an unreliable phenotypic diagnosis into a reliable genotypic test.
Solution Approach 2:
The patent introduces the DNM1 gene mutation status as an intermediary marker to diagnose EIC. Rather than directly observing the complex exercise-induced collapse phenotype, the invention uses the presence or absence of the G767T mutation as a surrogate indicator that reliably predicts EIC susceptibility.
2Measurement precision
If genetic testing is implemented, then accurate predisposition identification is achieved, but testing cost and complexity increase
Solution Approach 1:
The patent extracts only the critical diagnostic element (the G767T mutation in the DNM1 gene) from the complex EIC phenotype. By focusing solely on this specific nucleotide substitution, the invention achieves high detection accuracy while simplifying the testing approach compared to comprehensive phenotypic evaluation.
Solution Approach 2:
The patent changes the detection parameter from phenotypic observation (exercise performance, coordination) to genotypic parameter (nucleotide sequence at position 767). This parameter transformation enables precise identification of predisposition through molecular methods with high specificity and sensitivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate identification of dogs predisposed to EIC, facilitating informed breeding decisions and personalized therapy based on genetic profiles, reducing the incidence of the condition through genetic testing.
Implementation Method 1
performing a hybridization reaction between the nucleic acid sample and a probe or probes that are capable of hybridizing to the allele
Implementation Method 2
amplification steps are by polymerase chain reaction (PCR)
Implementation Method 3
determining the electrophoretic mobility of the allele or a component thereof
Data Source
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AI summary
The present invention relates to diagnosing Canine Exercise-Induced Collapse (EIC).