Canola 45H37 Variety Breeding via Marker-Assisted Selection

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Solution Overview

Problem

Conventional plant breeding methods face challenges in predicting and replicating desirable traits in canola varieties due to the unpredictability of genetic combinations and the lack of control at the DNA level, leading to high research costs and time-consuming development of superior canola varieties.

Innovation Solution

The development of a novel Brassica napus variety, 45H37, which incorporates cytoplasmic or nuclear factors for male sterility, herbicide tolerance, and resistance to diseases and insects, along with methods for introducing transgenes and locus conversions to enhance traits such as yield and disease resistance, allowing for controlled pollination and hybrid production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional plant breeding methods are used to develop new canola varieties, then various desirable traits can be combined in a single variety, but the process is time-consuming and costly due to unpredictability of genetic combinations and lack of control at the DNA level

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidbreeding development time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies parameter changes by moving from conventional phenotypic selection to genotypic selection using molecular markers. This changes the fundamental parameter of trait detection from observable characteristics to DNA-level markers, enabling precise tracking and combination of desirable traits while significantly reducing breeding time and cost.

Inventive Principle:
Principle #35Parameter changes

2Adaptability or versatility

If traditional plant breeding methods are used to develop new canola varieties, then various desirable traits can be combined in a single variety, but research costs become high due to unpredictability and lack of control

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidbreeding process cost
Core Design Contradiction:
Adaptability or versatilityVSEase of manufacture

Solution Approach 1:

The patent implements feedback mechanisms through molecular marker-assisted selection, where DNA markers provide continuous feedback on the genetic composition of breeding lines. This allows breeders to make informed decisions at each generation, tracking the accumulation of desirable traits and eliminating unwanted combinations, thereby reducing research costs and improving breeding efficiency.

Inventive Principle:
Principle #23Feedback

3Ease of operation

If male sterility factors are introduced into canola plants, then controlled pollination and hybrid production are enabled, but the plant requires additional genetic components

Engineering Contradiction:
Improvecontrolled pollinationVSAvoidgenetic composition
Core Design Contradiction:
Ease of operationVSDevice complexity

Solution Approach 1:

The patent applies the extraction principle by isolating and utilizing specific male sterility genes (such as thecms15 gene) from their original genetic background. This allows the sterility trait to be introduced into canola varieties independently, enabling controlled pollination without requiring complex entire-genome transformations, thus simplifying the overall genetic modification process.

Inventive Principle:
Principle #2Taking out (Extraction)

Data Source

PatentUS10440910B2Canola variety 45H37
Publication Date: 2019.10.15 PIONEER HI BREED INTERNATIONAL INC

AI summary

Provided is a canola variety designated 45H37 and seed, plants and plant parts thereof produced from a cross of inbred varieties. Methods for producing a canola variety comprise crossing canola variety 45H37 with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 45H37 through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby are described. Canola variety 45H37, the seed, the plant produced from the seed, plant parts and variants, mutants, and minor modifications of canola variety 45H37 are disclosed.