Canola DN040244A Breeding via Marker-Assisted Selection

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Solution Overview

Problem

The development of new canola cultivars is a time-consuming and unpredictable process due to the complexity of inheritance and the need for precise genetic combinations, making it challenging to produce cultivars with desired traits such as high yield, disease resistance, and nutritional quality consistently.

Innovation Solution

The development of the canola cultivar DN040244A, which is a high oleic, low linolenic acid cultivar resistant to Blackleg and Fusarium wilt, with integrated herbicide tolerance and improved nutritional profile, achieved through traditional breeding and dihaploid methodology, along with genetic engineering techniques to introduce desired traits like imidazolinone resistance and enhanced oil quality.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop new canola cultivars, then genetic diversity and trait combinations can be achieved, but the process becomes time-consuming and unpredictable

Engineering Contradiction:
Improvetrait combination capabilityVSAvoidbreeding cycle duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by creating and maintaining a pre-characterized germplasm collection with known genetic traits and marker profiles. This allows breeders to select parental lines with desired characteristics before crossing, significantly reducing the time required to develop new cultivars with specific trait combinations while maintaining genetic diversity.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent implements feedback through molecular marker-assisted selection during the breeding process. By monitoring genetic markers in intermediate generations, breeders can track the inheritance of desired traits and make informed selection decisions, accelerating the breeding cycle and improving predictability of outcome traits.

Inventive Principle:
Principle #23Feedback

2Productivity

If multiple desirable traits are combined in a single cultivar, then agricultural productivity and market value improve, but the complexity of achieving precise genetic combinations increases

Engineering Contradiction:
Improveagricultural outputVSAvoidgenetic combination complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by breaking down the complex task of combining multiple traits into manageable segments using molecular markers. Each desired trait is associated with specific marker profiles that can be tracked independently through generations, allowing systematic combination of multiple traits without overwhelming complexity.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent uses parameter changes by shifting from phenotypic selection to genotypic selection based on molecular marker profiles. This changes the selection parameter from observable traits to genetic markers, enabling more precise and efficient combination of multiple desirable traits in single cultivars.

Inventive Principle:
Principle #35Parameter changes

3Measurement precision

If molecular marker profiles are used to characterize germplasm, then selection precision improves, but the initial characterization process becomes more complex

Engineering Contradiction:
Improvetrait identification accuracyVSAvoidcharacterization process complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent applies preliminary action by performing comprehensive molecular marker characterization on germplasm collections before breeding operations. This initial investment in characterization creates a reusable database that simplifies subsequent selection processes, improving measurement precision without repeating the complex characterization work.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS8563810B2Canola cultivar DN040244A
Publication Date: 2013.10.22 AGRIGENETICS INC

AI summary

The present invention relates to a new and distinctive canola cultivar, designated DN040244A. Also included are seeds of canola cultivar DN040244A, to the plants, or plant parts, of canola DN040244A and to methods for producing a canola plant produced by crossing the canola DN040244A with itself or another canola cultivar, and the creation of variants by mutagenesis or transformation of canola DN040244A.