Canola DN040839 Breeding via Dhaploid Technology

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Solution Overview

Problem

The development of new canola cultivars is a time-consuming and unpredictable process due to the complexity of inheritance and the need for precise selection of genetically superior plants, which requires extensive research and resources, and existing cultivars may lack desirable traits such as high yield, disease resistance, and improved nutritional quality.

Innovation Solution

The introduction of the canola cultivar DN040839, which is developed through traditional breeding and dihaploid methodology, incorporating traits like high oleic acid content, imidazolinone resistance, blackleg resistance, and enhanced nutritional quality, along with methods for producing regenerable cells for tissue culture and introducing desired traits through genetic engineering or backcrossing.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop new canola cultivars, then genetic diversity and trait combination are improved, but the breeding process becomes time-consuming and unpredictable

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding process duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by creating dihaploid plants (plants with half the normal chromosome number) that can be rapidly doubled to produce homozygous lines. This preliminary step of creating dihaploids through controlled pollination and embryo rescue allows breeders to obtain genetically stable lines in just 2-3 years instead of the traditional 8-12 years, directly resolving the time loss contradiction while maintaining trait combination versatility.

Inventive Principle:
Principle #10Preliminary action

2Reliability

If extensive selection and evaluation of breeding lines are performed, then genetic superiority and trait stability are improved, but research and resource requirements increase

Engineering Contradiction:
Improvetrait stabilityVSAvoidresearch resources
Core Design Contradiction:
ReliabilityVSQuantity of substance

Solution Approach 1:

The patent applies the taking out principle by extracting and isolating specific desirable traits (such as blackleg resistance, yield characteristics, and oil composition) into defined breeding objectives. By using dihaploid technology to create genetically uniform lines, the method extracts genetic variability and presents it in a controlled manner, requiring fewer replication studies and resource-intensive evaluations to confirm trait stability, thus reducing research resource requirements while maintaining reliability.

Inventive Principle:
Principle #2Taking out (Extraction)

3Stability of the object's composition

If multiple breeding cycles and generations are used to stabilize traits, then cultivar uniformity and performance consistency are improved, but the development timeline and resource investment increase

Engineering Contradiction:
Improvecultivar uniformityVSAvoidbreeding efficiency
Core Design Contradiction:
Stability of the object's compositionVSProductivity

Solution Approach 1:

The patent applies parameter changes by fundamentally altering the chromosomal parameter of the breeding material - creating dihaploid plants with half the normal chromosome number (n=19 instead of 2n=38). This parameter change allows for rapid chromosome doubling to produce completely homozygous lines in a single generation, achieving cultivar uniformity without requiring multiple traditional breeding cycles. The result is stable, uniform cultivars developed in 2-3 years instead of 8-12 years, dramatically improving breeding efficiency while maintaining composition stability.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS7723578B2Canola cultivar DN040839
Publication Date: 2010.05.25 CORTEVA AGRISCIENCE LLC

AI summary

The present invention relates to a new and distinctive canola cultivar, designated DN040839. Also included are seeds of canola cultivar DN040839, to the plants, or plant parts, of canola DN040839 and to methods for producing a canola plant produced by crossing the canola DN040839 with itself or another canola cultivar, and the creation of variants by mutagenesis or transformation of canola DN040839.