Canola NS8063 Line Marker-Assisted Selection Breeding

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Solution Overview

Problem

Current canola breeding techniques face challenges in developing high-quality varieties with low erucic acid and glucosinolate content, requiring extensive effort and unpredictability in genetic combinations, and there is a need for efficient methods to introduce desirable traits like male sterility and disease resistance.

Innovation Solution

The development of a novel Brassica napus line, NS8063, which can be crossed with other Brassica plants to introduce male sterility and other traits through cytoplasmic conversion and backcrossing, utilizing molecular markers for marker-assisted selection, and employing techniques like cytoplasmic male sterility systems for hybrid production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Manufacturing precision

If traditional canola breeding techniques are used to develop high-quality varieties with low erucic acid and glucosinolate content, then the desired quality traits can be achieved, but the breeding process requires extensive effort and time with unpredictable genetic combinations

Engineering Contradiction:
Improvequality traits (low erucic acid and glucosinolate content)VSAvoidbreeding process duration
Core Design Contradiction:
Manufacturing precisionVSLoss of time

Solution Approach 1:

The patent develops and utilizes molecular markers that are预先 identified and linked to specific quality traits (low erucic acid and glucosinolate content) before the breeding process begins. These markers allow breeders to screen and select desirable genetic combinations early in the breeding program, rather than waiting for phenotypic expression later, thereby reducing the overall breeding time and increasing predictability of achieving the desired quality traits

Inventive Principle:
Principle #10Preliminary action

2Adaptability or versatility

If traditional breeding methods are used to introduce desirable traits like male sterility and disease resistance, then these traits can be incorporated into canola varieties, but the process is complex and time-consuming

Engineering Contradiction:
Improvedesirable traits (male sterility and disease resistance)VSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent employs molecular markers as intermediary tools that bridge the gap between traditional phenotypic selection and the desired genetic traits. These markers serve as reliable indicators that allow breeders to track and select for multiple desirable traits (male sterility, disease resistance, quality characteristics) simultaneously through a simplified marker-assisted selection process, reducing the complexity of traditional multi-trait breeding programs

Inventive Principle:
Principle #24Intermediary (Mediator)

3Manufacturing precision

If extensive breeding efforts are made to achieve specific quality characteristics, then high-quality canola varieties can be produced, but the genetic combinations remain unpredictable

Engineering Contradiction:
Improvequality characteristics (erucic acid and glucosinolate content)VSAvoidgenetic combination predictability
Core Design Contradiction:
Manufacturing precisionVSReliability

Solution Approach 1:

The patent implements a feedback mechanism through marker-assisted selection where molecular markers provide real-time information about the genetic composition of breeding lines. This feedback allows breeders to make informed decisions at each generation, selecting only those plants that carry the desired genetic combinations for low erucic acid and glucosinolate content, thereby increasing the reliability and predictability of achieving the target quality characteristics

Inventive Principle:
Principle #23Feedback

Data Source

PatentUS11160229B2Canola inbred NS8063
Publication Date: 2021.11.02 PIONEER HI BREED INTERNATIONAL INC

AI summary

A novel canola variety designated NS8063 and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety NS8063 with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into NS8063 through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety NS8063 or a locus conversion of NS8063 with another canola variety.